Document Detail

Ceramide in lipid particles enhances heparan sulfate proteoglycan and low density lipoprotein receptor-related protein-mediated uptake by macrophages.
MedLine Citation:
PMID:  15044445     Owner:  NLM     Status:  MEDLINE    
Arterial wall sphingomyelinase (SMase) has been proposed to be involved in atherogenesis. SMase modification of lipoproteins has been shown to occur in atherosclerotic lesions and to facilitate their uptake by macrophages and foam cell formation. To investigate the mechanism of macrophage uptake enhanced by SMase, we prepared lipid emulsions containing sphingomyelin (SM) or ceramide (CER) as model particles of lipoproteins. SMase remarkably increased the uptake of SM-containing emulsions by J774 macrophages without apolipoproteins. The emulsion uptake was negatively correlated with the degree of particle aggregation by pretreatment with SMase, whereas the uptake of CER-containing emulsions was significantly larger than SM-containing emulsions, indicating that enhancement of uptake is due to the generation of CER molecules in particles but not to the aggregation by SMase. Heparan sulfate proteoglycans (HSPGs) and low density lipoprotein receptor-related protein (LRP) were crucial for CER-enhanced emulsion uptake, because heparin or lactoferrin inhibited the emulsion uptake. Confocal microscopy also showed that SMase promoted both binding and internalization of emulsions by J774 macrophages, which were almost abolished by lactoferrin. Apolipoprotein E further increased the uptake of CER-containing emulsions compared with SM-containing emulsions. These findings suggest the generation of CER in lipoproteins by SMase facilitates the macrophage uptake via HSPG and LRP pathways and plays a crucial role in foam cell formation. Thus, CER may act as an important atherogenic molecule.
Shin-Ya Morita; Misa Kawabe; Atsushi Sakurai; Keiichirou Okuhira; Aline Vertut-Doï; Minoru Nakano; Tetsurou Handa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-03-24
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  279     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-05-31     Completed Date:  2004-07-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  24355-61     Citation Subset:  IM    
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
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MeSH Terms
Anticoagulants / pharmacology
Apolipoproteins E / chemistry,  metabolism
Cell Line
Ceramides / metabolism*
Foam Cells / metabolism
Heparan Sulfate Proteoglycans / metabolism*
Heparin / chemistry,  pharmacology
LDL-Receptor Related Protein 1 / metabolism*
Lactoferrin / chemistry,  pharmacology
Lipid Metabolism*
Lipoproteins / metabolism
Macrophages / metabolism*
Microscopy, Confocal
Time Factors
Reg. No./Substance:
0/Anticoagulants; 0/Apolipoproteins E; 0/Ceramides; 0/Heparan Sulfate Proteoglycans; 0/LDL-Receptor Related Protein 1; 0/Lactoferrin; 0/Lipoproteins; 9005-49-6/Heparin

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