Document Detail


Ceramic cells for high pressure NMR spectroscopy of proteins.
MedLine Citation:
PMID:  20359919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Application of high pressure to biological macromolecules can be used to find new structural states with a smaller specific volume of the system. High pressure NMR spectroscopy is a most promising analytical tool for the study of these states at atomic resolution. High pressure quartz cells are difficult to handle, high quality sapphire high pressure cells are difficult to obtain commercially. In this work, we describe the use of high pressure ceramic cells produced from yttrium stabilized ZrO(2) that are capable of resisting pressures up to 200 MPa. Since the new cells should also be usable in the easily damageable cryoprobes a completely new autoclave for these cells has been constructed, including an improved method for pressure transmission, an integrated safety jacket, a displacement body, and a fast self-closing emergency valve.
Authors:
Markus Beck Erlach; Claudia E Munte; Werner Kremer; Rainer Hartl; Dörte Rochelt; Dieter Niesner; Hans Robert Kalbitzer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-23
Journal Detail:
Title:  Journal of magnetic resonance (San Diego, Calif. : 1997)     Volume:  204     ISSN:  1096-0856     ISO Abbreviation:  J. Magn. Reson.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-21     Completed Date:  2010-09-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9707935     Medline TA:  J Magn Reson     Country:  United States    
Other Details:
Languages:  eng     Pagination:  196-9     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Biophysics, University of Regensburg, Universitätsstrasse 31, D-93040 Regensburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Ceramics / chemistry*
Equipment Design
Equipment Failure Analysis
Magnetic Resonance Spectroscopy / instrumentation*
Pressure
Proteins / analysis*,  chemistry*
Specimen Handling / instrumentation*
Chemical
Reg. No./Substance:
0/Ceramics; 0/Proteins

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