Document Detail


Cep57, a NEDD1-binding pericentriolar material component, is essential for spindle pole integrity.
MedLine Citation:
PMID:  22508265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Formation of a bipolar spindle is indispensable for faithful chromosome segregation and cell division. Spindle integrity is largely dependent on the centrosome and the microtubule network. Centrosome protein Cep57 can bundle microtubules in mammalian cells. Its related protein (Cep57R) in Xenopus was characterized as a stabilization factor for microtubule-kinetochore attachment. Here we show that Cep57 is a pericentriolar material (PCM) component. Its interaction with NEDD1 is necessary for the centrosome localization of Cep57. Depletion of Cep57 leads to unaligned chromosomes and a multipolar spindle, which is induced by PCM fragmentation. In the absence of Cep57, centrosome microtubule array assembly activity is weakened, and the spindle length and microtubule density decrease. As a spindle microtubule-binding protein, Cep57 is also responsible for the proper organization of the spindle microtubule and localization of spindle pole focusing proteins. Collectively, these results suggest that Cep57, as a NEDD1-binding centrosome component, could function as a spindle pole- and microtubule-stabilizing factor for establishing robust spindle architecture.
Authors:
Qixi Wu; Runsheng He; Haining Zhou; Albert C H Yu; Bo Zhang; Junlin Teng; Jianguo Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-17
Journal Detail:
Title:  Cell research     Volume:  22     ISSN:  1748-7838     ISO Abbreviation:  Cell Res.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-03     Completed Date:  2013-01-09     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  9425763     Medline TA:  Cell Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  1390-401     Citation Subset:  IM    
Affiliation:
The State Key Laboratory of Biomembrane and Membrane Bioengineering and The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Centrosome / metabolism
Cricetinae
Cricetulus
HeLa Cells
Humans
Mice
Microtubule-Associated Proteins / antagonists & inhibitors,  genetics,  metabolism*
Mitotic Spindle Apparatus / metabolism*
Nuclear Proteins / antagonists & inhibitors,  genetics,  metabolism*
Protein Binding
RNA Interference
RNA, Small Interfering / metabolism
Xenopus / metabolism
Chemical
Reg. No./Substance:
0/CEP57 protein, human; 0/Microtubule-Associated Proteins; 0/NEDD1 protein, human; 0/Nuclear Proteins; 0/RNA, Small Interfering
Comments/Corrections

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