Document Detail

Centrosome and microtubules behavior in the cytoplasts.
MedLine Citation:
PMID:  7671218     Owner:  NLM     Status:  MEDLINE    
After enucleation of PK (pig kidney embryo) cells using cytochalasin D centrioles remained approximately in 80% of cytoplasts. Some cytoplasts retained a single centriole. 14-16 h after enucleation large secondary lysosomes and lipid droplets were evident around the centrosome of many cytoplasts. In part of the cytoplasts replicating centrioles were found 16 h after enucleation. Ouabain treatment (1 mM, 30 min) of the cytoplasts resulted in the appearance of mainly perpendicular orientation of mother centrioles relative to the substrate surface. Reconstruction of microtubule pattern around the centrosome showed that a total of approximately 15 microtubules were attached in the centrosome of normal cells and twice more than that in cytoplasts. Microtubules in the cytoplasts were more resistant to nocodazole induced depolymerization. We suggest that microtubule instability is modulated by regulatory effector that is under direct nucleus control.
L A Gorgidze; I A Vorobjev
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of submicroscopic cytology and pathology     Volume:  27     ISSN:  1122-9497     ISO Abbreviation:  J. Submicrosc. Cytol. Pathol.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-10-17     Completed Date:  1995-10-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8804312     Medline TA:  J Submicrosc Cytol Pathol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  381-9     Citation Subset:  IM    
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
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MeSH Terms
Cell Nucleus / drug effects
Cells, Cultured
Centrosome / ultrastructure*
Cytochalasin D / pharmacology
Fluorescent Antibody Technique
Microscopy, Electron
Microtubules / drug effects,  ultrastructure*
Nocodazole / pharmacology
Reg. No./Substance:
22144-77-0/Cytochalasin D; 31430-18-9/Nocodazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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