| Centrosome dynamics in early embryos of Caenorhabditis elegans. | |
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MedLine Citation:
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PMID: 9739076 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The early Caenorhabditis elegans embryo divides with a stereotyped pattern of cleavages to produce cells that vary in developmental potential. Differences in cleavage plane orientation arise between the anterior and posterior cells of the 2-cell embryo as a result of asymmetries in centrosome positioning. Mechanisms that position centrosomes are thought to involve interactions between microtubules and the cortex, however, these mechanisms remain poorly defined. Interestingly, in the early embryo the shape of the centrosome predicts its subsequent movement. We have used rhodamine-tubulin and live imaging techniques to study the development of asymmetries in centrosome morphology and positioning. In contrast to studies using fixed embryos, our images provide a detailed characterization of the dynamics of centrosome flattening. In addition, our observations of centrosome behavior in vivo challenge previous assumptions regarding centrosome separation by illustrating that centrosome flattening and daughter centrosome separation are distinct processes, and by revealing that nascent daughter centrosomes may become separated from the nucleus. Finally, we provide evidence that the midbody specifies a region of the cortex that directs rotational alignment of the centrosome-nucleus complex and that the process is likely to involve multiple interactions between microtubules and the cortex; the process of alignment involves oscillations and overshoots, suggesting a multiplicity of cortical sites that interact with microtubules. |
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Authors:
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H H Keating; J G White |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cell science Volume: 111 ( Pt 20) ISSN: 0021-9533 ISO Abbreviation: J. Cell. Sci. Publication Date: 1998 Oct |
Date Detail:
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Created Date: 1998-12-22 Completed Date: 1998-12-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 3027-33 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Biology and Department of Anatomy, University of Wisconsin-Madison, Madison, WI 53706, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Caenorhabditis elegans / embryology* Cell Division* Cell Nucleus / ultrastructure Centrosome / metabolism, ultrastructure* Embryo, Nonmammalian / ultrastructure* Image Processing, Computer-Assisted Immunohistochemistry Interphase Microtubules / ultrastructure Mitotic Spindle Apparatus / ultrastructure Rhodamines Tubulin / analysis |
| Grant Support | |
ID/Acronym/Agency:
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GM17922/GM/NIGMS NIH HHS; GM52454/GM/NIGMS NIH HHS; RR00570/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Rhodamines; 0/Tubulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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