Document Detail


Centrosome duplication in mammalian somatic cells requires E2F and Cdk2-cyclin A.
MedLine Citation:
PMID:  10559879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Centrosome duplication is a key requirement for bipolar spindle formation and correct segregation of chromosomes during cell division. In a manner highly reminiscent of DNA replication, the centrosome must be duplicated once, and only once, in each cell cycle. How centrosome duplication is regulated and coordinated with other cell-cycle functions remains poorly understood. Here, we have established a centrosome duplication assay using mammalian somatic cells. We show that centrosome duplication requires the activation of E2F transcription factors and Cdk2-cyclin A activity.
Authors:
P Meraldi; J Lukas; A M Fry; J Bartek; E A Nigg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nature cell biology     Volume:  1     ISSN:  1465-7392     ISO Abbreviation:  Nat. Cell Biol.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-12-16     Completed Date:  1999-12-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100890575     Medline TA:  Nat Cell Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  88-93     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, University of Geneva, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
CDC2-CDC28 Kinases*
CHO Cells
Carrier Proteins*
Cell Cycle / physiology*
Cell Cycle Proteins / metabolism
Centrosome / physiology*,  ultrastructure*
Cricetinae
Cyclin A / metabolism*
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinases / metabolism*
DNA Replication*
DNA-Binding Proteins*
E2F Transcription Factors
Mammals
Protein-Serine-Threonine Kinases / metabolism*
Recombinant Proteins / metabolism
Retinoblastoma-Binding Protein 1
Transcription Factors / metabolism*
Transfection
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Cell Cycle Proteins; 0/Cyclin A; 0/DNA-Binding Proteins; 0/E2F Transcription Factors; 0/Recombinant Proteins; 0/Retinoblastoma-Binding Protein 1; 0/Transcription Factors; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.22/CDC2-CDC28 Kinases; EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/Cyclin-Dependent Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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