| Centrosome duplication in mammalian somatic cells requires E2F and Cdk2-cyclin A. | |
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MedLine Citation:
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PMID: 10559879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Centrosome duplication is a key requirement for bipolar spindle formation and correct segregation of chromosomes during cell division. In a manner highly reminiscent of DNA replication, the centrosome must be duplicated once, and only once, in each cell cycle. How centrosome duplication is regulated and coordinated with other cell-cycle functions remains poorly understood. Here, we have established a centrosome duplication assay using mammalian somatic cells. We show that centrosome duplication requires the activation of E2F transcription factors and Cdk2-cyclin A activity. |
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Authors:
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P Meraldi; J Lukas; A M Fry; J Bartek; E A Nigg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nature cell biology Volume: 1 ISSN: 1465-7392 ISO Abbreviation: Nat. Cell Biol. Publication Date: 1999 Jun |
Date Detail:
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Created Date: 1999-12-16 Completed Date: 1999-12-16 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 100890575 Medline TA: Nat Cell Biol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 88-93 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, University of Geneva, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals CDC2-CDC28 Kinases* CHO Cells Carrier Proteins* Cell Cycle / physiology* Cell Cycle Proteins / metabolism Centrosome / physiology*, ultrastructure* Cricetinae Cyclin A / metabolism* Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinases / metabolism* DNA Replication* DNA-Binding Proteins* E2F Transcription Factors Mammals Protein-Serine-Threonine Kinases / metabolism* Recombinant Proteins / metabolism Retinoblastoma-Binding Protein 1 Transcription Factors / metabolism* Transfection |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Cell Cycle Proteins; 0/Cyclin A; 0/DNA-Binding Proteins; 0/E2F Transcription Factors; 0/Recombinant Proteins; 0/Retinoblastoma-Binding Protein 1; 0/Transcription Factors; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.22/CDC2-CDC28 Kinases; EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/Cyclin-Dependent Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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