Document Detail

Centrosome development in early mouse embryos as defined by an autoantibody against pericentriolar material.
MedLine Citation:
PMID:  6652679     Owner:  NLM     Status:  MEDLINE    
A human autoantibody from a schleroderma patient was found to immunostain interphase and mitotic centrosomes in a variety of vertebrate cells. Electron microscopic immunocytochemistry localized this antigen in dense pericentriolar material (PCM) surrounding the centrioles. The meiotic spindle of the mouse egg has no centriole but it exhibited a broad PCM band at each pole. This pattern was also found from the first through fourth mitotic divisions. During this time PCM was found assembled at a single locus in the cell and exclusively in mitotic cells; it was not observable in interphase cells. In the blastocyst, only polar trophoblast cells had characteristic centrosomes throughout the cell cycle. Results suggest PCM can exist, disperse, and reorganize during the cell cycle independently of the centriole, and its distribution in the embryo differs in cells having different fates.
P D Calarco-Gillam; M C Siebert; R Hubble; T Mitchison; M Kirschner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  35     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-02-15     Completed Date:  1984-02-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  621-9     Citation Subset:  IM    
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MeSH Terms
Autoantibodies / immunology
Centrioles / immunology*
Cleavage Stage, Ovum / ultrastructure*
Mice / embryology
Mitotic Spindle Apparatus / ultrastructure*
Organoids / immunology*
Scleroderma, Systemic / immunology
Grant Support
Reg. No./Substance:

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