| Centromeric DNA hypomethylation as an epigenetic signature discriminates between germ and somatic cell lineages. | |
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MedLine Citation:
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PMID: 17964565 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Germ cells have unique features strikingly distinguishable from somatic cells. The functional divergence between these two cell lineages has been postulated to result from epigenetic mechanisms. Here we show that the chromosomal centric and pericentric (C/P) regions in male and female germline cells are specifically DNA-hypomethylated, despite the hypermethylation status in somatic cells. In multipotent germline stem cells, the C/P region was initially hypomethylated and then shifted to the hypermethylation status during differentiation into somatic lineage in vitro. Moreover, the somatic-type hypermethylation pattern was maintained in the somatic cell-derived nuclear transfer embryos throughout preimplantation development. These results imply that the identity of germ cell lineage may be warranted by the hypomethylation status of the C/P region as an epigenetic signature. |
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Authors:
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Kazuo Yamagata; Taiga Yamazaki; Hiromi Miki; Narumi Ogonuki; Kimiko Inoue; Atsuo Ogura; Tadashi Baba |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-09-29 |
Journal Detail:
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Title: Developmental biology Volume: 312 ISSN: 1095-564X ISO Abbreviation: Dev. Biol. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-12-06 Completed Date: 2007-12-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 419-26 Citation Subset: IM |
Affiliation:
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Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Lineage* Centromere / genetics*, metabolism* CpG Islands DNA Methylation* DNA, Satellite / metabolism Embryo Implantation Embryo, Mammalian / metabolism Epigenesis, Genetic* Female Fertilization in Vitro Germ Cells / cytology*, metabolism* Humans Male Mice Nuclear Reprogramming Nuclear Transfer Techniques Organ Specificity Ovum / cytology, metabolism Sequence Analysis, DNA Spermatozoa / cytology, metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA, Satellite |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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