Document Detail


Centromeric DNA hypomethylation as an epigenetic signature discriminates between germ and somatic cell lineages.
MedLine Citation:
PMID:  17964565     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Germ cells have unique features strikingly distinguishable from somatic cells. The functional divergence between these two cell lineages has been postulated to result from epigenetic mechanisms. Here we show that the chromosomal centric and pericentric (C/P) regions in male and female germline cells are specifically DNA-hypomethylated, despite the hypermethylation status in somatic cells. In multipotent germline stem cells, the C/P region was initially hypomethylated and then shifted to the hypermethylation status during differentiation into somatic lineage in vitro. Moreover, the somatic-type hypermethylation pattern was maintained in the somatic cell-derived nuclear transfer embryos throughout preimplantation development. These results imply that the identity of germ cell lineage may be warranted by the hypomethylation status of the C/P region as an epigenetic signature.
Authors:
Kazuo Yamagata; Taiga Yamazaki; Hiromi Miki; Narumi Ogonuki; Kimiko Inoue; Atsuo Ogura; Tadashi Baba
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-09-29
Journal Detail:
Title:  Developmental biology     Volume:  312     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-06     Completed Date:  2007-12-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  419-26     Citation Subset:  IM    
Affiliation:
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Lineage*
Centromere / genetics*,  metabolism*
CpG Islands
DNA Methylation*
DNA, Satellite / metabolism
Embryo Implantation
Embryo, Mammalian / metabolism
Epigenesis, Genetic*
Female
Fertilization in Vitro
Germ Cells / cytology*,  metabolism*
Humans
Male
Mice
Nuclear Reprogramming
Nuclear Transfer Techniques
Organ Specificity
Ovum / cytology,  metabolism
Sequence Analysis, DNA
Spermatozoa / cytology,  metabolism
Chemical
Reg. No./Substance:
0/DNA, Satellite

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