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Centromere and telomere movements during early meiotic prophase of mouse and man are associated with the onset of chromosome pairing.
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MedLine Citation:
PMID:  8794855     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The preconditions and early steps of meiotic chromosome pairing were studied by fluorescence in situ hybridization (FISH) with chromosome-specific DNA probes to mouse and human testis tissue sections. Premeiotic pairing of homologous chromosomes was not detected in spermatogonia of the two species. FISH with centromere- and telomere-specific DNA probes in combination with immunostaining (IS) of synaptonemal complex (SC) proteins to testis sections of prepuberal mice at days 4-12 post partum was performed to study sequentially the meiotic pairing process. Movements of centromeres and then telomeres to the nuclear envelope, and of telomeres along the nuclear envelope leading to the formation of a chromosomal bouquet were detected during mouse prophase. At the bouquet stage, pairing of a mouse chromosome-8-specific probe was observed. SC-IS and simultaneous telomere FISH revealed that axial element proteins appear as large aggregates in mouse meiocytes when telomeres are attached to the nuclear envelope. Axial element formation initiates during tight telomere clustering and transverse filament-IS indicated the initiation of synapsis during this stage. Comparison of telomere and centromere distribution patterns of mouse and human meiocytes revealed movements of centromeres and then telomeres to the nuclear envelope and subsequent bouquet formation as conserved motifs of the pairing process. Chromosome painting in human spermatogonia revealed compacted, largely mutually exclusive chromosome territories. The territories developed into long, thin threads at the onset of meiotic prophase. Based on these results a unified model of the pairing process is proposed.
Authors:
H Scherthan; S Weich; H Schwegler; C Heyting; M Härle; T Cremer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of cell biology     Volume:  134     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-10-24     Completed Date:  1996-10-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1109-25     Citation Subset:  IM    
Affiliation:
Department of Human Genetics and Human Biology, University of Kaiserslautern, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / physiology
Centromere / physiology*
Chromosomes / physiology
Chromosomes, Human, Pair 1 / physiology
Humans
In Situ Hybridization, Fluorescence
Male
Meiosis / physiology*
Mice
Mice, Inbred BALB C
Movement
Nuclear Envelope / physiology
Prophase / physiology*
Spermatogonia / physiology
Staining and Labeling
Synaptonemal Complex / physiology
Telomere / physiology*
Testis / cytology,  physiology
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 9 Year: 1996
Volume: 134 Issue: 5
First Page: 1109 Last Page: 1125
ID: 2120985
Publisher Id: 96387320
PubMed Id: 8794855

Centromere and telomere movements during early meiotic prophase of mouse and man are associated with the onset of chromosome pairing


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