| Centriole reduplication during prolonged interphase requires procentriole maturation governed by Plk1. | |
| | |
MedLine Citation:
|
PMID: 20656208 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Supernumerary centrioles lead to abnormal mitosis, which in turn promotes tumorigenesis. Thus, centriole duplication must be coordinated with the cell cycle to ensure that the number of centrioles in the cell doubles precisely during each cell cycle. However, in some transformed cells, centrioles undergo multiple rounds of duplication (reduplication) during prolonged interphase. Mechanisms responsible for centriole reduplication are poorly understood. Here, we report that centrioles reduplicate consistently in cancerous and nontransformed human cells during G2 arrests and that this reduplication requires the activity of Polo-like kinase 1 (Plk1). We also find that a cell's ability to reduplicate centrioles during S arrests depends on the presence of activated (Thr210-phosphorylated) Plk1 at the centrosome. In the absence of activated Plk1, nascent procentrioles remain associated with mother centrioles, which prevents centriole reduplication. In contrast, if Plk1(pT210) appears at the centrosome, procentrioles mature, disengage from mother centrioles, and ultimately duplicate. Plk1 activity is not required for the assembly of procentrioles, however. Thus, the role of Plk1 is to coordinate the centriole duplication cycle with the cell cycle. Activation of Plk1 during late S/G2 induces procentriole maturation, and after this point, the centriole cycle can be completed autonomously, even in the absence of cell-cycle progression. |
| | |
Authors:
|
Jadranka Loncarek; Polla Hergert; Alexey Khodjakov |
Related Documents
:
|
18160328 - Clioquinol inhibits peroxide-mediated toxicity through up-regulation of phosphoinositol... 20016278 - Ligand-driven activation of the notch pathway in t-all and solid tumors: why not(ch)? |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-06-17 |
Journal Detail:
|
Title: Current biology : CB Volume: 20 ISSN: 1879-0445 ISO Abbreviation: Curr. Biol. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-07-26 Completed Date: 2010-11-26 Revised Date: 2011-11-02 |
Medline Journal Info:
|
Nlm Unique ID: 9107782 Medline TA: Curr Biol Country: England |
Other Details:
|
Languages: eng Pagination: 1277-82 Citation Subset: IM |
Copyright Information:
|
2010 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Division of Translational Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201-0509, USA. loncarek@wadsworth.org |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Cycle Proteins
/
metabolism* Centrioles / physiology* Hela Cells Humans Interphase / physiology* Microscopy, Confocal Phosphorylation Protein-Serine-Threonine Kinases / metabolism* Proto-Oncogene Proteins / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
GM59363/GM/NIGMS NIH HHS; R01 GM059363-12/GM/NIGMS NIH HHS; R01 GM059363-13/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cell Cycle Proteins; 0/Proto-Oncogene Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/polo-like kinase 1 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Polarisation vision: beetles see circularly polarised light.
Next Document: Plasmodium falciparum accompanied the human expansion out of Africa.