Document Detail


Centrifugal elutriation as a means of cell cycle phase separation and synchronisation.
MedLine Citation:
PMID:  17623921     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability to purify cells in specific phases of the cell cycle in sufficient quantities for biochemical analysis or to obtain a "synchronized" population of living cells enriched in a particular phase is of great value for studying a wide range of cell cycle processes. Transformed tumour cell lines, such as DT40, can be arrested and released from specific points in the cell cycle using drugs which inhibit DNA or protein synthesis or interfere with mitotic spindle function, however such drugs are frequently toxic and may themselves perturb the cell cycle process or phenomenon under study. Centrifugal elutriation provides a means of separating an unperturbed culture of living cells into highly enriched G1-, S-, and G2/ M-phase fractions. The resulting cell fractions can be analysed directly for protein and mRNA expression or returned to culture and their subsequent progression through the cell cycle monitored by flow cytometry, microscopy, or other techniques.
Authors:
David A F Gillespie; Catarina Henriques
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Sub-cellular biochemistry     Volume:  40     ISSN:  0306-0225     ISO Abbreviation:  Subcell. Biochem.     Publication Date:  2006  
Date Detail:
Created Date:  2007-07-12     Completed Date:  2007-07-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0316571     Medline TA:  Subcell Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  359-61     Citation Subset:  IM    
Affiliation:
CR-UK Beatson Laboratories, Beatson Institute for Cancer Research, Garscube Estate, Bearsden, Glasgow G61 1BD.
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MeSH Terms
Descriptor/Qualifier:
Animals
B-Lymphocytes / cytology
Cell Cycle*
Cell Line
Centrifugation
Chickens

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