| Central importance of immunoglobulin A in host defense against Giardia spp. | |
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MedLine Citation:
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PMID: 11748158 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The protozoan pathogen Giardia is an important cause of parasitic diarrheal disease worldwide. It colonizes the lumen of the small intestine, suggesting that effective host defenses must act luminally. Immunoglobulin A (IgA) antibodies are presumed to be important for controlling Giardia infection, but direct evidence for this function is lacking. B-cell-independent effector mechanisms also exist and may be equally important for antigiardial host defense. To determine the importance of the immunoglobulin isotypes that are transported into the intestinal lumen, IgA and IgM, for antigiardial host defense, we infected gene-targeted mice lacking IgA-expressing B-cells, IgM-secreting B-cells, or all B-cells as controls with Giardia muris or Giardia lamblia GS/M-83-H7. We found that IgA-deficient mice could not eradicate either G. muris or G. lamblia infection, demonstrating that IgA is required for their clearance. Furthermore, although neither B-cell-deficient nor IgA-deficient mice could clear G. muris infections, IgA-deficient mice controlled infection significantly better than B-cell-deficient mice, suggesting the existence of B-cell-dependent but IgA-independent antigiardial defenses. In contrast, mice deficient for secreted IgM antibodies cleared G. muris infection normally, indicating that they have no unique functions in antigiardial host defense. These data, together with the finding that B-cell-deficient mice have some, albeit limited, residual capacity to control G. muris infection, show that IgA-dependent host defenses are central for eradicating Giardia spp. Moreover, B-cell-dependent but IgA-independent and B-cell-independent antigiardial host defenses exist but are less important for controlling infection. |
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Authors:
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T Dianne Langford; Michael P Housley; Marianne Boes; Jianzhu Chen; Martin F Kagnoff; Frances D Gillin; Lars Eckmann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Infection and immunity Volume: 70 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 2002 Jan |
Date Detail:
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Created Date: 2001-12-18 Completed Date: 2002-01-14 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 11-8 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of California, San Diego, La Jolla, California 92093-0623, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Protozoan / immunology* B-Lymphocytes / immunology* Disease Models, Animal Giardia / immunology Giardia lamblia Giardiasis / immunology*, prevention & control Immunity, Active / immunology Immunoglobulin A / immunology* Immunoglobulin M / immunology Intestine, Small / immunology Mice Mice, Inbred C57BL Mice, Knockout |
| Grant Support | |
ID/Acronym/Agency:
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DK35108/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Protozoan; 0/Immunoglobulin A; 0/Immunoglobulin M |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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