Document Detail


Cementum, root dentin and bone extracts stimulate chemotactic behavior in cells from periodontal tissue.
MedLine Citation:
PMID:  9114496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper, we used the extracts from cementum, bone, dentin and enamel, and compared the chemotactic behavior of human periodontal ligament (HPDL) cells, human gingival fibroblasts (HGF) and human alveolar bone (HAB) cells using modified Boyden chambers. The extracts, obtained using 4 M guanidine HCl ("G" extract), from cementum, root dentin, and bone had greater chemotactic effects than all the 4 M guanidine HCl/0.5 M EDTA extracts ("E" extract). HPDL cells and HGF exhibited higher chemotactic behavior than osteoblast-like HAB cells in response to cementum and root dentin "G" extracts. On the other hand, HAB cells showed the highest migratory activity in response to the bone "G" extract. The chemotactic activities of these extracts were reduced by heat- and trypsin-treatment. These results suggest that proteinaceous chemotactic factors exist in the cementum, root dentin and bone, and they appear to regulate the migration and orientation of HPDL cells, HGF and HAB cells during periodontal wound healing.
Authors:
Y Ogata; N Niisato; K Moriwaki; Y Yokota; S Furuyama; H Sugiya
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  116     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-06-05     Completed Date:  1997-06-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  359-65     Citation Subset:  IM    
Affiliation:
Department of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alveolar Process / cytology,  drug effects
Bone and Bones / chemistry*
Chemotactic Factors / pharmacology
Chemotaxis / drug effects
Dental Cementum / chemistry*
Dentin / chemistry*
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel / methods
Fibroblasts
Gingiva / cytology,  drug effects
Hot Temperature
Humans
Periodontal Ligament / cytology,  drug effects
Periodontium / cytology*,  drug effects*
Tissue Extracts / pharmacology
Trypsin / chemistry
Chemical
Reg. No./Substance:
0/Chemotactic Factors; 0/Tissue Extracts; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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