Document Detail


Cellulose promotes extracellular assembly of Clostridium cellulovorans cellulosomes.
MedLine Citation:
PMID:  7961457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cellulosome synthesis by Clostridium cellulovorans was investigated by growing the cells in media containing different carbon sources. Supernatant from cells grown with cellobiose contained no cellulosomes and only the free forms of cellulosomal major subunits CbpA, P100, and P70 and the minor subunits with enzymatic activity. Supernatant from cells grown on pebble-milled cellulose and Avicel contained cellulosomes capable of degrading crystalline cellulose. Supernatants from cells grown with cellobiose, pebble-milled cellulose, and Avicel contained about the same amount of carboxymethyl cellulase activity. Although the supernatant from the medium containing cellobiose did not initially contain active cellulosomes, the addition of crystalline cellulose to the cell-free supernatant fraction converted the free major forms to cellulosomes with the ability to degrade crystalline cellulose. The binding of P100 and P70 to crystalline cellulose was dependent on their attachment to the endoglucanase-binding domains of CbpA. These data strongly indicate that crystalline cellulose promotes cellulosome assembly.
Authors:
Y Matano; J S Park; M A Goldstein; R H Doi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  176     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1994-12-16     Completed Date:  1994-12-16     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6952-6     Citation Subset:  IM    
Affiliation:
Section of Molecular and Cellular Biology, University of California, Davis 95616.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / metabolism*
Binding Sites
Carrier Proteins*
Cellobiose / metabolism
Cellulase / biosynthesis*
Cellulose / metabolism*
Clostridium / enzymology*
Protein Conformation
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Carrier Proteins; 147258-12-6/cellulose binding protein A, Clostridium cellulovorans; 16462-44-5/Cellobiose; 9004-34-6/Cellulose; EC 3.2.1.4/Cellulase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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