Document Detail


Cellular uptake and phototoxicity of bioconjugated fluorescent nanodiamonds.
MedLine Citation:
PMID:  22138396     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Fluorescent nanodiamonds (FNDs) show great potential in effectively targeted imaging of cancer cells. In this study, we investigated the cellular uptake of transferrin (Tf) conjugated FNDs and evaluated the phototoxicity of FNDs internalized by cells. Through an analysis of flow cytometry, the cellular uptake of FND-Tf reached saturation value with half-life time of approximately 0.8h at concentration of 10μg/mL through the pathway of receptor-mediated endocytosis. An examination of the phototoxicity of internalized FND-Tf particles by using a 532nm laser revealed that the FND-treated cells could be killed, whereas untreated cells survived under the same irradiative condition. With the support of Raman spectra, nanodiamond clusters showed that particle temperature increased under irradiation. We suggest that the enhanced cellular killing was attributed to the conversion of light absorbed by FND particles into thermal energy. Our results demonstrated that bioconjugated FNDs could be a specific probe for fluorescence imaging and kill cancer cells selectively by photothermal effect.
Authors:
Mao-Feng Weng; I-Chun Cho; Su-Yu Chiang; Huan Niu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-25
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  -     ISSN:  1090-2104     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-12-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
Affiliation:
Isotope Application Division, Institute of Nuclear Energy Research, Taoyuan 32546, Taiwan.
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