Document Detail

Cellular uptake and ex vivo urothelial penetration by oligodeoxynucleotides for optimizing treatment of transitional cell carcinoma.
MedLine Citation:
PMID:  18980158     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate cellular uptake and urothelial penetration of oligodeoxynucleotides (ODNs) in transitional cell carcinoma (TCC) cell lines and in a porcine ex vivo model, respectively.
STUDY DESIGN: A panel of human TCC cell lines (RT 112, HT 1197 and UM-UC3) were exposed tofluorescein-labeled ODNs. Transfection rates were assessed byfluorescence microscopy and fluorescence-activated cell sorting (FACS). Intravesical treatment with ODNs was performed in a porcine ex vivo model. Urothelial penetration was evaluated using fluorescence microscopy of cryosections.
RESULTS: Treatment with ODNs provided transfection rates of at least 96.8% of TCC cells, irrespective of use of a transfection agent. Effective urothelial penetration by ODNs was detected when compared with controls (p = 0.0325). The addition of a liposomal transfection agent significantly increased the penetration depth, allowing affection of deep urothelial cell layers (p = 0.0082).
CONCLUSION: High transfection rates of ODNs can be achieved in TCC cells. Urothelial penetration of ODNs was observed down to the deepest cell layers when a transfection agent is added, suggesting a high potential for complementing the chemoresection effects on residual tumor areas during intravesical therapy of non-muscle-invasive TCC.
Christian Bolenz; Lutz Trojan; Ute Gabriel; Patrick Honeck; Gunnar Wendt-Nordahl; Axel Schaaf; Peter Alken; Maurice Stephan Michel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology     Volume:  30     ISSN:  0884-6812     ISO Abbreviation:  Anal. Quant. Cytol. Histol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-11-04     Completed Date:  2008-11-26     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  8506819     Medline TA:  Anal Quant Cytol Histol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  265-73     Citation Subset:  IM    
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MeSH Terms
Carcinoma, Transitional Cell / metabolism,  pathology,  therapy*
Cell Line, Tumor
Disease Models, Animal
Fluorescent Dyes
Genetic Therapy / methods*
Oligodeoxyribonucleotides / pharmacokinetics*
Transfection / methods
Urinary Bladder Neoplasms / metabolism,  pathology,  therapy*
Urothelium / metabolism*
Reg. No./Substance:
0/Fluorescent Dyes; 0/Oligodeoxyribonucleotides; I223NX31W9/Fluorescein-5-isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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