Document Detail


Cellular uptake and ex vivo urothelial penetration by oligodeoxynucleotides for optimizing treatment of transitional cell carcinoma.
MedLine Citation:
PMID:  18980158     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To evaluate cellular uptake and urothelial penetration of oligodeoxynucleotides (ODNs) in transitional cell carcinoma (TCC) cell lines and in a porcine ex vivo model, respectively.
STUDY DESIGN: A panel of human TCC cell lines (RT 112, HT 1197 and UM-UC3) were exposed tofluorescein-labeled ODNs. Transfection rates were assessed byfluorescence microscopy and fluorescence-activated cell sorting (FACS). Intravesical treatment with ODNs was performed in a porcine ex vivo model. Urothelial penetration was evaluated using fluorescence microscopy of cryosections.
RESULTS: Treatment with ODNs provided transfection rates of at least 96.8% of TCC cells, irrespective of use of a transfection agent. Effective urothelial penetration by ODNs was detected when compared with controls (p = 0.0325). The addition of a liposomal transfection agent significantly increased the penetration depth, allowing affection of deep urothelial cell layers (p = 0.0082).
CONCLUSION: High transfection rates of ODNs can be achieved in TCC cells. Urothelial penetration of ODNs was observed down to the deepest cell layers when a transfection agent is added, suggesting a high potential for complementing the chemoresection effects on residual tumor areas during intravesical therapy of non-muscle-invasive TCC.
Authors:
Christian Bolenz; Lutz Trojan; Ute Gabriel; Patrick Honeck; Gunnar Wendt-Nordahl; Axel Schaaf; Peter Alken; Maurice Stephan Michel
Related Documents :
8003028 - Delivery of plasmid dna to glial cells using ph-sensitive immunoliposomes.
16878698 - Mammalian cell microinjection assay to study the function of rho family guanosine triph...
11023198 - A cell surface-displayed anti-c-myc single-chain antibody: new perspectives for the gen...
1409618 - Translocation of apolipoprotein b across the endoplasmic reticulum is blocked in a nonh...
21901628 - Human fibroblasts for large-scale "omics" investigations of atm gene function.
24932308 - Gastric cancer cell growth and epithelial-mesenchymal transition are inhibited by γ-sec...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology     Volume:  30     ISSN:  0884-6812     ISO Abbreviation:  Anal. Quant. Cytol. Histol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-11-04     Completed Date:  2008-11-26     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  8506819     Medline TA:  Anal Quant Cytol Histol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  265-73     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Transitional Cell / metabolism,  pathology,  therapy*
Cell Line, Tumor
Disease Models, Animal
Fluorescein-5-isothiocyanate
Fluorescent Dyes
Genetic Therapy / methods*
Humans
Oligodeoxyribonucleotides / pharmacokinetics*
Swine
Transfection / methods
Urinary Bladder Neoplasms / metabolism,  pathology,  therapy*
Urothelium / metabolism*
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Oligodeoxyribonucleotides; I223NX31W9/Fluorescein-5-isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Overdiagnosis of bladder carcinoma.
Next Document:  Argyrophilic nucleolar organizer region counts in exfoliative cytology of buccal mucosa from opium a...