Document Detail


Cellular uptake and cytotoxic potential of respirable bentonite particles with different quartz contents and chemical modifications in human lung fibroblasts.
MedLine Citation:
PMID:  16059726     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Considering the biological reactivity of pure quartz in lung cells, there is a strong interest to clarify the cellular effects of respirable siliceous dusts, like bentonites. In the present study, we investigated the cellular uptake and the cytotoxic potential of bentonite particles (Ø< 10 microm) with an alpha-quartz content of up to 6% and different chemical modifications (activation: alkaline, acidic, organic) in human lung fibroblasts (IMR90). Additionally, the ability of the particles to induce apoptosis in IMR90-cells and the hemolytic activity was tested. All bentonite samples were tested for endotoxins with the in vitro-Pyrogen test and were found to be negative. Cellular uptake of particles by IMR90-cells was studied by transmission electron microscopy (TEM). Cytotoxicity was analyzed in IMR90-cells by determination of viable cells using flow cytometry and by measuring of the cell respiratory activity. Induced apoptotic cells were detected by AnnexinV/Propidiumiodide-staining and gel electrophoresis. Our results demonstrate that activated bentonite particles are better taken up by IMR90-cells than untreated (native) bentonite particles. Also, activated bentonite particles with a quartz content of 5-6% were more cytotoxic than untreated bentonites or bentonites with a quartz content lower than 4%. The bentonite samples induced necrotic as well as apoptotic cell death. In general, bentonites showed a high membrane-damaging potential shown as hemolytic activity in human erythrocytes. We conclude that cellular effects of bentonite particles in human lung cells are enhanced after chemical treatment of the particles. The cytotoxic potential of the different bentonites is primarily characterized by a strong lysis of the cell membrane.
Authors:
Stefan Geh; Raif Yücel; Rodger Duffin; Catrin Albrecht; Paul J A Borm; Lorenz Armbruster; Monika Raulf-Heimsoth; Thomas Brüning; Eik Hoffmann; Albert W Rettenmeier; Elke Dopp
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-07-30
Journal Detail:
Title:  Archives of toxicology     Volume:  80     ISSN:  0340-5761     ISO Abbreviation:  Arch. Toxicol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-19     Completed Date:  2006-08-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417615     Medline TA:  Arch Toxicol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  98-106     Citation Subset:  IM    
Affiliation:
Institute of Hygiene and Occupational Medicine, University Hospital, Hufelandstrasse 55, 45122 Essen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Air Pollutants / toxicity*
Apoptosis / drug effects*
Bentonite* / chemistry,  toxicity
Cell Survival / drug effects
Cells, Cultured
Fibroblasts / drug effects*,  ultrastructure
Flow Cytometry
Humans
Lung / cytology,  drug effects*,  ultrastructure
Particle Size
Quartz* / analysis
Surface Properties
X-Ray Diffraction
Chemical
Reg. No./Substance:
0/Air Pollutants; 1302-78-9/Bentonite; 14808-60-7/Quartz

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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