| Cellular uptake and cytotoxic potential of respirable bentonite particles with different quartz contents and chemical modifications in human lung fibroblasts. | |
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MedLine Citation:
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PMID: 16059726 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Considering the biological reactivity of pure quartz in lung cells, there is a strong interest to clarify the cellular effects of respirable siliceous dusts, like bentonites. In the present study, we investigated the cellular uptake and the cytotoxic potential of bentonite particles (Ø< 10 microm) with an alpha-quartz content of up to 6% and different chemical modifications (activation: alkaline, acidic, organic) in human lung fibroblasts (IMR90). Additionally, the ability of the particles to induce apoptosis in IMR90-cells and the hemolytic activity was tested. All bentonite samples were tested for endotoxins with the in vitro-Pyrogen test and were found to be negative. Cellular uptake of particles by IMR90-cells was studied by transmission electron microscopy (TEM). Cytotoxicity was analyzed in IMR90-cells by determination of viable cells using flow cytometry and by measuring of the cell respiratory activity. Induced apoptotic cells were detected by AnnexinV/Propidiumiodide-staining and gel electrophoresis. Our results demonstrate that activated bentonite particles are better taken up by IMR90-cells than untreated (native) bentonite particles. Also, activated bentonite particles with a quartz content of 5-6% were more cytotoxic than untreated bentonites or bentonites with a quartz content lower than 4%. The bentonite samples induced necrotic as well as apoptotic cell death. In general, bentonites showed a high membrane-damaging potential shown as hemolytic activity in human erythrocytes. We conclude that cellular effects of bentonite particles in human lung cells are enhanced after chemical treatment of the particles. The cytotoxic potential of the different bentonites is primarily characterized by a strong lysis of the cell membrane. |
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Authors:
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Stefan Geh; Raif Yücel; Rodger Duffin; Catrin Albrecht; Paul J A Borm; Lorenz Armbruster; Monika Raulf-Heimsoth; Thomas Brüning; Eik Hoffmann; Albert W Rettenmeier; Elke Dopp |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-07-30 |
Journal Detail:
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Title: Archives of toxicology Volume: 80 ISSN: 0340-5761 ISO Abbreviation: Arch. Toxicol. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-01-19 Completed Date: 2006-08-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0417615 Medline TA: Arch Toxicol Country: Germany |
Other Details:
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Languages: eng Pagination: 98-106 Citation Subset: IM |
Affiliation:
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Institute of Hygiene and Occupational Medicine, University Hospital, Hufelandstrasse 55, 45122 Essen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Air Pollutants
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toxicity* Apoptosis / drug effects* Bentonite* / chemistry, toxicity Cell Survival / drug effects Cells, Cultured Fibroblasts / drug effects*, ultrastructure Flow Cytometry Humans Lung / cytology, drug effects*, ultrastructure Particle Size Quartz* / analysis Surface Properties X-Ray Diffraction |
| Chemical | |
Reg. No./Substance:
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0/Air Pollutants; 1302-78-9/Bentonite; 14808-60-7/Quartz |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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