Document Detail


Cellular tropism of human enterovirus D species serotypes EV-94, EV-70, and EV-68 in vitro: implications for pathogenesis.
MedLine Citation:
PMID:  20872722     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Enterovirus 94 (EV-94) is an enterovirus serotype described recently which, together with EV-68 and EV-70, forms human enterovirus D species. This study investigates the seroprevalences of these three serotypes and their abilities to infect, replicate, and damage cell types considered to be essential for enterovirus-induced diseases. The cell types studied included human leukocyte cell lines, primary endothelial cells, and pancreatic islets. High prevalence of neutralizing antibodies against EV-68 and EV-94 was found in the Finnish population. The virus strains studied had wide leukocyte tropism. EV-94 and EV-68 were able to produce infectious progeny in leukocyte cell lines with monocytic, granulocytic, T-cell, or B-cell characteristics. EV-94 and EV-70 were capable of infecting primary human umbilical vein endothelial cells, whereas EV-68 had only marginal progeny production and did not induce cytopathic effects in these cells. Intriguingly, EV-94 was able to damage pancreatic islet β-cells, to infect, replicate, and cause necrosis in human pancreatic islets, and to induce proinflammatory and chemoattractive cytokine expression in endothelial cells. These results suggest that HEV-D viruses may be more prevalent than has been thought previously, and they provide in vitro evidence that EV-94 may be a potent pathogen and should be considered a potentially diabetogenic enterovirus type.
Authors:
Teemu Smura; Petri Ylipaasto; Päivi Klemola; Svetlana Kaijalainen; Lauri Kyllönen; Valeria Sordi; Lorenzo Piemonti; Merja Roivainen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical virology     Volume:  82     ISSN:  1096-9071     ISO Abbreviation:  J. Med. Virol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2011-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705876     Medline TA:  J Med Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1940-9     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley-Liss, Inc.
Affiliation:
Intestinal Viruses Unit, Division of Health Protection, Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), Helsinki, Finland. teemu.smura@thl.fi
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cells, Cultured
Diabetes Mellitus, Type 1 / pathology,  virology
Endothelial Cells / virology*
Enterovirus D, Human / classification,  pathogenicity*
Enterovirus Infections / epidemiology*,  pathology,  virology
Humans
Islets of Langerhans / virology*
Leukocytes / virology*
Seroepidemiologic Studies
Serotyping
Species Specificity
Umbilical Veins / cytology
Virus Replication

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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