| Cellular tropism of human enterovirus D species serotypes EV-94, EV-70, and EV-68 in vitro: implications for pathogenesis. | |
| | |
MedLine Citation:
|
PMID: 20872722 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Enterovirus 94 (EV-94) is an enterovirus serotype described recently which, together with EV-68 and EV-70, forms human enterovirus D species. This study investigates the seroprevalences of these three serotypes and their abilities to infect, replicate, and damage cell types considered to be essential for enterovirus-induced diseases. The cell types studied included human leukocyte cell lines, primary endothelial cells, and pancreatic islets. High prevalence of neutralizing antibodies against EV-68 and EV-94 was found in the Finnish population. The virus strains studied had wide leukocyte tropism. EV-94 and EV-68 were able to produce infectious progeny in leukocyte cell lines with monocytic, granulocytic, T-cell, or B-cell characteristics. EV-94 and EV-70 were capable of infecting primary human umbilical vein endothelial cells, whereas EV-68 had only marginal progeny production and did not induce cytopathic effects in these cells. Intriguingly, EV-94 was able to damage pancreatic islet β-cells, to infect, replicate, and cause necrosis in human pancreatic islets, and to induce proinflammatory and chemoattractive cytokine expression in endothelial cells. These results suggest that HEV-D viruses may be more prevalent than has been thought previously, and they provide in vitro evidence that EV-94 may be a potent pathogen and should be considered a potentially diabetogenic enterovirus type. |
| | |
Authors:
|
Teemu Smura; Petri Ylipaasto; Päivi Klemola; Svetlana Kaijalainen; Lauri Kyllönen; Valeria Sordi; Lorenzo Piemonti; Merja Roivainen |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of medical virology Volume: 82 ISSN: 1096-9071 ISO Abbreviation: J. Med. Virol. Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-09-27 Completed Date: 2011-01-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7705876 Medline TA: J Med Virol Country: United States |
Other Details:
|
Languages: eng Pagination: 1940-9 Citation Subset: IM |
Copyright Information:
|
© 2010 Wiley-Liss, Inc. |
Affiliation:
|
Intestinal Viruses Unit, Division of Health Protection, Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), Helsinki, Finland. teemu.smura@thl.fi |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Cells, Cultured Diabetes Mellitus, Type 1 / pathology, virology Endothelial Cells / virology* Enterovirus D, Human / classification, pathogenicity* Enterovirus Infections / epidemiology*, pathology, virology Humans Islets of Langerhans / virology* Leukocytes / virology* Seroepidemiologic Studies Serotyping Species Specificity Umbilical Veins / cytology Virus Replication |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Prevalence of low-risk and high-risk types of human papillomavirus and other risk factors for HPV in...
Next Document: Enterovirus infection induces cytokine and chemokine expression in insulin-producing cells.