Document Detail


Cellular and thylakoid-membrane phospholipids of Chlamydomonas reinhardtii 137+.
MedLine Citation:
PMID:  7299293     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The phospholipids of phototrophically-cultured Chlamydomonas reinhardtii 137+ have been quantitated at the cellular and thylakoid membrane levels. The alga contains three major phospholipids, phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and phosphatidylcholine (PC), which together constitute about 14% of the cellular polar lipid complement. PG is the only phospholipid in an analytical fraction of thylakoid membrane isolated from the alga, and represents about 9% of the membrane's total polar lipid. Slightly more than half the cellular PG is localized in the photosynthetic lamellae. Hexadecanoic and octadecanoic fatty acids make up over 70% of the acyl groups esterified to these phospholipids, PG being the most highly unsaturated of the three. Differences in fatty acid profile between cellular and thylakoid PG, as well as the presence of PE and PC in the alga but not in its thylakoid, are indicative of a strict biogenetic regulation of the specific types and species of phospholipid associated with the photosynthetic membrane.
Authors:
D R Janero; R Barrnett
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  22     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1981 Sep 
Date Detail:
Created Date:  1982-01-28     Completed Date:  1982-01-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1126-30     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Cell Membrane / analysis
Chlamydomonas / analysis*
Chromatography, Thin Layer
Fatty Acids / analysis
Glycolipids / analysis
Intracellular Membranes / analysis*
Membrane Lipids / analysis*
Phosphatidylcholines / analysis
Phosphatidylethanolamines / analysis
Phosphatidylglycerols / analysis
Phospholipids / analysis*
Grant Support
ID/Acronym/Agency:
AM-03688/AM/NIADDK NIH HHS; GM-07223/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Glycolipids; 0/Membrane Lipids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phosphatidylglycerols; 0/Phospholipids

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