Document Detail


Cellular and subcellular distribution of iron in the lamina propria of rat duodenum.
MedLine Citation:
PMID:  6467343     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cellular and subcellular distribution of iron in the lamina propria of rat duodenum was studied after a single i.p. injection of iron dextran, using electron microscopy and peroxidase cytochemistry. X-ray spectrum microanalysis was used for positive identification of iron. Iron-containing particles (IP) were found in the cytoplasm of three cell types, viz. macrophages, pericytic reticular cells and sheathing fibrocytes. IP-containing organelles in lamina propria cells were more heterogeneous compared to absorptive cells and, in addition, some differences were noted in the subcellular distribution of IP in the 3 cell types. A common denominator in these 3 cell types was the presence of endogenous peroxidase, also shared by Kupffer cells which are known to be involved in iron storage. Peroxidase activity was absent in absorptive epithelial cells. It is hypothesized that the cells of the lamina propria, like Kupffer cells, may be the site of storage of excess iron absorbed, releasing iron upon demand and migrating into the lumen to prevent iron overload. In this fashion they may regulate the exchange of iron with the environment. The presence of peroxidase in these as well as Kupffer cells, and its absence in absorptive cells also raises the possibility that this enzyme may be related to certain aspects of iron storing process.
Authors:
M Aoki; M Ito; M Tavassoli
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell and tissue research     Volume:  236     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  1984  
Date Detail:
Created Date:  1984-10-19     Completed Date:  1984-10-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  685-92     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Duodenum / analysis*,  enzymology,  ultrastructure
Intestinal Mucosa / analysis*,  ultrastructure
Iron / analysis*
Macrophages / analysis
Male
Peroxidases / analysis
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
AM-30142/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
7439-89-6/Iron; EC 1.11.1.-/Peroxidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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