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Cellular and subcellular distribution of a cAMP-regulated prestalk protein and prespore protein in Dictyostelium discoideum: a study on the ontogeny of prestalk and prespore cells.
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MedLine Citation:
PMID:  3023394     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have analyzed a developmentally and spatially regulated prestalk-specific gene and a prespore-specific gene from Dictyostelium. The prestalk gene, pst-cathepsin, encodes a protein highly homologous to the lysosomal cysteine proteinases cathepsin H and cathepsin B. The prespore gene encodes a protein with some homology to the anti-bacterial toxin crambin and has been designated beejin. Using the lambda gtll system, we have made polyclonal antibodies directed against a portion of the protein encoded by pst-cathepsin and other antibodies directed against the beejin protein. Both antibodies stain single bands on Western blots. By immunofluorescence and Western blots, pst-cathepsin is not present in vegetative cells or developing cells during the first approximately 10 h of development. It then appears with a punctate distribution in a subset of developing cells. Beejin is detected only after approximately 15 h of development, also in a subset of cells. Pst-cathepsin is distributed in the anterior approximately 1/10 of migrating slugs and on the peripheral posterior surfaces of slugs. Beejin is distributed in the posterior region of slugs. Expression of both pst-cathepsin and beejin can be induced in subsets of isolated cultured cells by a combination of conditioned medium and extracellular cAMP in agreement with the regulation of the mRNAs encoding these proteins. We have used the antibodies as markers for cell type to examine the ontogeny and the spatial distribution of prestalk and prespore cells throughout multicellular development. Our findings suggest that prestalk cell differentiation is independent of position within the aggregate and that the spatial localization of prestalk cells within the multicellular aggregate arises from sorting of the prestalk cells after their induction. We have also found a class of cell in developing aggregates that contains neither the prestalk nor the prespore markers.
Authors:
R H Gomer; S Datta; R A Firtel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  103     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1987-01-21     Completed Date:  1987-01-21     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1999-2015     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / diagnostic use
Cathepsins / genetics,  immunology
Cell Compartmentation
Cell Differentiation
Cyclic AMP / physiology
Dictyostelium / cytology*,  genetics
Fungal Proteins / genetics,  immunology,  metabolism
Gene Expression Regulation
Immunologic Techniques
Protozoan Proteins*
Spores, Fungal
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Fungal Proteins; 0/Protozoan Proteins; 107565-44-6/COTB protein, Dictyostelium discoideum; 60-92-4/Cyclic AMP; EC 3.4.-/Cathepsins
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Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 11 Year: 1986
Volume: 103 Issue: 5
First Page: 1999 Last Page: 2015
ID: 2114392
Publisher Id: 87057653
PubMed Id: 3023394

Cellular and subcellular distribution of a cAMP-regulated prestalk protein and prespore protein in Dictyostelium discoideum: a study on the ontogeny of prestalk and prespore cells


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