Document Detail

Cellular retinoic acid-binding proteins are essential for hindbrain patterning and signal robustness in zebrafish.
MedLine Citation:
PMID:  22619388     Owner:  NLM     Status:  MEDLINE    
The vitamin A derivative retinoic acid (RA) is a morphogen that patterns the anterior-posterior axis of the vertebrate hindbrain. Cellular retinoic acid-binding proteins (Crabps) transport RA within cells to both its nuclear receptors (RARs) and degrading enzymes (Cyp26s). However, mice lacking Crabps are viable, suggesting that Crabp functions are redundant with those of other fatty acid-binding proteins. Here we show that Crabps in zebrafish are essential for posterior patterning of the hindbrain and that they provide a key feedback mechanism that makes signaling robust as they are able to compensate for changes in RA production. Of the four zebrafish Crabps, Crabp2a is uniquely RA inducible and depletion or overexpression of Crabp2a makes embryos hypersensitive to exogenous RA. Computational models confirm that Crabp2a improves robustness within a narrow concentration range that optimizes a 'robustness index', integrating spatial information along the RA morphogen gradient. Exploration of signaling parameters in our models suggests that the ability of Crabp2a to transport RA to Cyp26 enzymes for degradation is a major factor in promoting robustness. These results demonstrate a previously unrecognized requirement for Crabps in RA signaling and hindbrain development, as well as a novel mechanism for stabilizing morphogen gradients despite genetic or environmental fluctuations in morphogen availability.
Anna Q Cai; Kelly Radtke; Angela Linville; Arthur D Lander; Qing Nie; Thomas F Schilling
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  139     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-23     Completed Date:  2012-08-07     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2150-5     Citation Subset:  IM    
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MeSH Terms
Body Patterning / drug effects,  genetics*
Gene Expression Regulation, Developmental / drug effects
Homeodomain Proteins / genetics,  metabolism
Models, Biological
Receptors, Retinoic Acid / genetics,  metabolism*
Rhombencephalon / drug effects,  embryology*,  metabolism
Signal Transduction / drug effects,  genetics*
Tretinoin / pharmacology
Zebrafish / embryology*,  genetics*
Zebrafish Proteins / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/Crabp2a protein, zebrafish; 0/Crabp2b protein, zebrafish; 0/Homeodomain Proteins; 0/Receptors, Retinoic Acid; 0/Zebrafish Proteins; 5688UTC01R/Tretinoin

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