| Cellular response to Mineral Trioxide Aggregate. | |
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MedLine Citation:
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PMID: 9759017 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This investigation studied the cytomorphology of osteoblasts in the presence of Mineral Trioxide Aggregate (MTA) and examined cytokine production. MTA and Intermediate Restorative Material (IRM) were prepared and placed in separate Petri dishes. Osteoblasts (cell-line MG-63), grown to confluence in Hams F12/Dulbecco's modified Eagle's medium, were seeded into the dishes, which were incubated for 1 to 7 days. The specimens were viewed by scanning electron microscopy. For cytokine evaluation, cells were grown either alone or in other dishes containing the test materials for 1 to 144 h. Media were removed for ELISA analysis of interleukin (IL)-1 alpha, IL-1 beta, IL-6, and macrophage colony-stimulating factor. Scanning electron microscopy revealed healthy cells in contact with MTA at 1 and 3 days; in contrast, cells in the presence of IRM appeared rounded. The ELISA assays revealed raised levels of all ILs at all periods when cells were grown in the presence of MTA; in contrast, cells grown alone or with IRM produced undetectable amounts. The macrophage colony-stimulating factor was produced by cells irrespective of the group. It seems that MTA offers a biologically active substrate for bone cells and stimulates IL production. |
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Authors:
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E T Koh; F McDonald; T R Pitt Ford; M Torabinejad |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of endodontics Volume: 24 ISSN: 0099-2399 ISO Abbreviation: J Endod Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1998-10-08 Completed Date: 1998-10-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7511484 Medline TA: J Endod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 543-7 Citation Subset: D |
Affiliation:
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Department of Conservative Dentistry, United Medical School, London, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aluminum Compounds
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pharmacology* Calcium Compounds / pharmacology* Cementogenesis Dental Cementum / drug effects*, metabolism Drug Combinations Enzyme-Linked Immunosorbent Assay Humans Interleukin-1 / biosynthesis Interleukin-6 / biosynthesis Macrophage Colony-Stimulating Factor / biosynthesis Methylmethacrylates / pharmacology Microscopy, Electron, Scanning Osteoblasts / drug effects*, metabolism Oxides / pharmacology* Root Canal Filling Materials / pharmacology* Silicates / pharmacology* Tumor Cells, Cultured Zinc Oxide-Eugenol Cement / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Aluminum Compounds; 0/Calcium Compounds; 0/Drug Combinations; 0/Interleukin-1; 0/Interleukin-6; 0/Methylmethacrylates; 0/Oxides; 0/Root Canal Filling Materials; 0/Silicates; 0/Zinc Oxide-Eugenol Cement; 0/mineral trioxide aggregate; 60318-33-4/IRM cement; 81627-83-0/Macrophage Colony-Stimulating Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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