| Cellular redox potential and hemoglobin S-glutathionylation in human and rat erythrocytes: A comparative study. | |
| | |
MedLine Citation:
|
PMID: 19963409 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The rat is commonly used to evaluate responses of red blood cells (RBCs) to oxidative stress. How closely the rat RBC model predicts the human RBC human response has not been well characterized. The objective of this study was to compare human and rat RBC responses to the thiol-specific oxidant tert-butylhydroperoxide by monitoring the intraerythrocyte glutathione redox potential and its correlation with hemoglobin S-glutathionylation. Changes in redox potential did not differ significantly between rat and human RBCs under the considered conditions, and both human and rat hemoglobins were apparently S-glutathionylated by a thiol-disulfide exchange mechanism with glutathione disulfide, though the extent of S-glutathionylation in rat erythrocytes was more than 10-fold higher than in human ones. On the contrary, human and rat hemoglobin S-glutathionylation differently correlated with redox potential for the glutathione redox couple, suggesting that the formation of S-glutathionylated hemoglobin was not simply a function of glutathione disulfide concentration or glutathione/glutathione disulfide ratio and that the content of reactive cysteines in hemoglobin beta globin can strongly influence intraerythrocyte glutathione metabolism and distribution between free and hemoglobin-bound forms. This study reveals fundamental physiological differences in rat and human RBCs because of differences in rat and human beta globin cysteine and reactivity, which can have important implications for the study of rat biology as a whole and for the use of rats as models for human beings under physiological and pathological circumstances and, therefore, highlights the need for caution when extrapolating rat responses to humans. |
| | |
Authors:
|
Graziano Colombo; Isabella Dalle-Donne; Daniela Giustarini; Nicoletta Gagliano; Nicola Portinaro; Roberto Colombo; Ranieri Rossi; Aldo Milzani |
Related Documents
:
|
9877199 - Glutathione conjugation of perchloroethene in subcellular fractions from rodent and hum... 496899 - The distribution of glutathione in the rat liver lobule. 2872029 - Cytosolic glutathione s-transferases in various rat tissues differ in stereoselectivity... 16800819 - Different susceptibility to peroxisome proliferator-induced hepatocarcinogenesis in rat... 17518349 - Evalution of toxicity of abcisic acid and gibberellic acid in rats: 50 days drinking wa... 8329859 - Age dependent different influence of carbon tetrachloride on biotransformation of xenob... 1142109 - The effect of acetazolamide and carbonic anhydrase inhibition on erythrocyte 2,3-diphos... 22037839 - Hypothyroid states mitigate the diabetes-induced reduction of calbindin d-28k, calretin... 7376199 - Distribution and excretion of 203hg2+ in rats after unitiol, spironolactone and polythi... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-06 |
Journal Detail:
|
Title: Blood cells, molecules & diseases Volume: 44 ISSN: 1096-0961 ISO Abbreviation: Blood Cells Mol. Dis. Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-02-26 Completed Date: 2010-05-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9509932 Medline TA: Blood Cells Mol Dis Country: United States |
Other Details:
|
Languages: eng Pagination: 133-9 Citation Subset: IM |
Copyright Information:
|
2009 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Biology, Universit? degli Studi di Milano, Milan, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Animals Erythrocytes / metabolism* Glutathione / metabolism* Glutathione Disulfide / metabolism Hemoglobin, Sickle / metabolism* Humans Middle Aged Oxidation-Reduction Oxidative Stress Rats Rats, Sprague-Dawley tert-Butylhydroperoxide / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Hemoglobin, Sickle; 27025-41-8/Glutathione Disulfide; 70-18-8/Glutathione; 75-91-2/tert-Butylhydroperoxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Conditionally replicating adenovirus therapy utilizing bone sialoprotein promoter (Ad-BSP-E1a) in an...
Next Document: Vaginal Yolk Sac Tumor Causing Infantile Hydrometra: Use of Multidetector-Row Computed Tomography.