Document Detail

Cellular radiation effects and hyperthermia cell cycle kinetics of radiation sensitive mutants of Saccharomyces cerevisiae after X-irradiation and hyperthermia.
MedLine Citation:
PMID:  6364198     Owner:  NLM     Status:  MEDLINE    
Radiosensitive mutants rad2, rad9, and rad51 of Saccharomyces cerevisiae were X-irradiated with 120 Gy or 60 Gy, heated at 50 degrees C for 30 min or treated with a combination of both and incubated in nutrient medium at 30 degrees C. Cell number, percentage of budding cells, and cell cycle progression were determined in 45-min intervals. Cell cycle kinetics were investigated by flow cytofluorometry. Hyperthermia leads mainly to a lengthening of G1, whereas X-rays arrest cells of the rad2 and rad9 mutant in G2 and the rad51--mutant additionaly in a state with DNA contents above G2. Cell division delay is influenced by oxygen in all strains but to a lesser extent in the rad2 mutant. The effect of the combined treatment appears to be merely additive in the rad2 and rad9 mutant while the rad51 mutant is sensitized to X-irradiation by hyperthermia. No selective action of hyperthermia on hypoxic cells was found.
R Fingerhut; J Kiefer; F Otto
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Radiation and environmental biophysics     Volume:  22     ISSN:  0301-634X     ISO Abbreviation:  Radiat Environ Biophys     Publication Date:  1983  
Date Detail:
Created Date:  1984-03-06     Completed Date:  1984-03-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0415677     Medline TA:  Radiat Environ Biophys     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  269-80     Citation Subset:  IM    
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MeSH Terms
Cell Cycle / radiation effects
DNA, Fungal / radiation effects
Hot Temperature*
Radiation Tolerance*
Saccharomyces cerevisiae / radiation effects*
Reg. No./Substance:
0/DNA, Fungal

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