Document Detail


Cellular protein differences between nontumorigenic Ad5 and tumorigenic Ad12 transformed mouse cells.
MedLine Citation:
PMID:  3727404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Murine fibroblasts transformed by adenovirus 12 (Ad12) show reduced class I major histocompatibility complex (MHC) antigen synthesis and form tumors in syngeneic mice whereas those transformed by adenovirus 5 (Ad5) show no alteration in class I antigen synthesis and do not form tumors (K. B. Eager, J. Williams, D. Breiding, S. Pan, B. Knowles, E. Appella, and R. P. Ricciardi (1985) Proc. Natl. Acad. Sci. USA 82, 5525-5529). Nearly 1500 metabolically labeled polypeptides from the Ad5 and Ad12 transformed cell lines as well as polypeptides from a nontransformed murine line of the same haplotype were compared by two-dimensional gel electrophoresis. In addition to the reduction of the class I H-2 transplantation antigens seen in the Ad12-transformed lines, we detect few but reproducible polypeptide differences between the tumorigenic and nontumorigenic cell lines.
Authors:
K B Eager; J A Sawicki; R P Ricciardi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virology     Volume:  152     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1986 Jul 
Date Detail:
Created Date:  1986-08-14     Completed Date:  1986-08-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  487-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae*
Animals
Cell Transformation, Neoplastic*
Cell Transformation, Viral*
Electrophoresis, Polyacrylamide Gel
Haploidy
Histocompatibility Antigens*
Mice
Molecular Weight
Grant Support
ID/Acronym/Agency:
CA-09171/CA/NCI NIH HHS; CA-29797/CA/NCI NIH HHS; HD-GM-16760/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Histocompatibility Antigens

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