| Cellular plasticity of inflammatory myeloid cells in the peritoneal foreign body response. | |
| | |
MedLine Citation:
|
PMID: 20008135 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Implantation of sterile foreign objects in the peritoneal cavity of an animal initiates an inflammatory response and results in encapsulation of the objects by bone marrow-derived cells. Over time, a multilayered tissue capsule develops with abundant myofibroblasts embedded in extracellular matrix. The present study used the transgenic MacGreen mouse to characterize the time-dependent accumulation of monocyte subsets and neutrophilic granulocytes in the inflammatory infiltrate and within the tissue capsule by their differential expression of the csf1r-EGFP transgene, F4/80, and Ly6C. As the tissue capsule developed, enhanced green fluorescent protein-positive cells changed from rounded to spindle-shaped morphology and began to co-express the myofibroblast marker alpha-smooth muscle actin. Expression increased with time: at day 14, 11.13 +/- 0.67% of tissue capsule cells co-expressed these markers, compared with 50.77 +/- 12.85% of cells at day 28. The importance of monocyte/macrophages in tissue capsule development was confirmed by clodronate-encapsulated liposome removal, which resulted in almost complete abrogation of capsule development. These results confirm the importance of monocyte/macrophages in the tissue response to sterile foreign objects implanted in the peritoneal cavity. In addition, the in vivo plasticity of peritoneal macrophages and their ability to transdifferentiate from a myeloid to mesenchymal phenotype is demonstrated. |
| | |
Authors:
|
Jane E Mooney; Barbara E Rolfe; Geoffrey W Osborne; David P Sester; Nico van Rooijen; Gordon R Campbell; David A Hume; Julie H Campbell |
Related Documents
:
|
10087425 - Pathogenesis of endometriosis: the role of peritoneal fluid. 12687185 - A study of peritoneal immunocompetent cells in external genital endometriosis. 6966615 - Depressed immunological defence mechanisms in mice with experimentally induced diabetes. 1940055 - Enhanced macrophage anti-microbial activity following dimethylnitrosamine exposure in v... 14511235 - Apoptosis induction by interleukin-2-activated cytotoxic lymphocytes in a squamous cell... 798635 - Preliminary studies of artificial immunization of rats against plasmodium berghei and a... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-11 |
Journal Detail:
|
Title: The American journal of pathology Volume: 176 ISSN: 1525-2191 ISO Abbreviation: Am. J. Pathol. Publication Date: 2010 Jan |
Date Detail:
|
Created Date: 2009-12-30 Completed Date: 2010-03-15 Revised Date: 2011-07-19 |
Medline Journal Info:
|
Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: United States |
Other Details:
|
Languages: eng Pagination: 369-80 Citation Subset: AIM; IM |
Affiliation:
|
Centre for Research in Vascular Biology, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, QLD 4072, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Movement Cell Shape Cell Transdifferentiation Female Fibroblasts / cytology Foreign Bodies / pathology Foreign-Body Reaction / pathology* Green Fluorescent Proteins / metabolism Implants, Experimental Macrophages / cytology Male Mice Myeloid Cells / pathology* Peritoneal Cavity / pathology* Peritoneal Lavage |
| Chemical | |
Reg. No./Substance:
|
0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Tissue factor-deficiency and protease activated receptor-1-deficiency reduce inflammation elicited b...
Next Document: WNT5A expression in ameloblastoma and its roles in regulating enamel epithelium tumorigenic behavior...