| Cellular pathways controlling integron cassette site folding. | |
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MedLine Citation:
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PMID: 20628355 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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By mobilizing small DNA units, integrons have a major function in the dissemination of antibiotic resistance among bacteria. The acquisition of gene cassettes occurs by recombination between the attI and attC sites catalysed by the IntI1 integron integrase. These recombination reactions use an unconventional mechanism involving a folded single-stranded attC site. We show that cellular bacterial processes delivering ssDNA, such as conjugation and replication, favour proper folding of the attC site. By developing a very sensitive in vivo assay, we also provide evidence that attC sites can recombine as cruciform structures by extrusion from double-stranded DNA. Moreover, we show an influence of DNA superhelicity on attC site extrusion in vitro and in vivo. We show that the proper folding of the attC site depends on both the propensity to form non-recombinogenic structures and the length of their variable terminal structures. These results draw the network of cell processes that regulate integron recombination. |
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Authors:
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Céline Loot; David Bikard; Anna Rachlin; Didier Mazel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-13 |
Journal Detail:
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Title: The EMBO journal Volume: 29 ISSN: 1460-2075 ISO Abbreviation: EMBO J. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-04 Completed Date: 2010-08-26 Revised Date: 2011-08-25 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: England |
Other Details:
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Languages: eng Pagination: 2623-34 Citation Subset: IM |
Affiliation:
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Institut Pasteur, Unité Plasticité du Génome Bactérien, Paris, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA
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chemistry* DNA Replication Integrons* Molecular Sequence Data Nucleic Acid Conformation* Recombination, Genetic |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
| Comments/Corrections | |
Erratum In:
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EMBO J. 2010 Nov 3;29(21):3745 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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