Document Detail

Cellular organization of normal mouse liver: a histological, quantitative immunocytochemical, and fine structural analysis.
MedLine Citation:
PMID:  19255771     Owner:  NLM     Status:  MEDLINE    
The cellular organization of normal mouse liver was studied using light and electron microscopy and quantitative immunocytochemical techniques. The general histological organization of the mouse liver is similar to livers of other mammalian species, with a lobular organization based on the distributions of portal areas and central venules. The parenchymal hepatocytes were detected with immunocytochemical techniques to recognize albumin or biotin containing cells. The macrophage Kupffer cells were identified with F4-80 immunocytochemistry, Ito stellate cells were identified with GFAP immunocytochemistry, and endothelial cells were labeled with the CD-34 antibody. Kupffer cells were labeled with intravascularly administered fluorescently labeled latex microspheres of both large (0.5 mum) and small (0.03 mum) diameters, while endothelial cells were labeled only with small diameter microspheres. Neither hepatocytes nor Ito stellate cells were labeled by intravascularly administered latex microspheres. The principal fine structural features of hepatocytes and non-parenchymal cells of mouse liver are similar to those reported for rat. Counts of immunocytochemically labeled cells with stained nuclei indicated that hepatocytes constituted approximately 52% of all labeled cells, Kupffer cells about 18%, Ito cells about 8%, and endothelial cells about 22% of all labeled cells. Approximately, 35% of the hepatocytes contained two nuclei; none of the Kupffer or Ito cells were double nucleated. The presence of canaliculi and a bile duct system appear similar to that reported for other species. The cellular organization of the mouse liver is quite similar to that of other mammalian species, confirming that the mouse presents a useful animal model for studies of liver structure and function.
Janie L Baratta; Anthony Ngo; Bryan Lopez; Natasha Kasabwalla; Kenneth J Longmuir; Richard T Robertson
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-03-03
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  131     ISSN:  1432-119X     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-21     Completed Date:  2009-07-31     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  713-26     Citation Subset:  IM    
Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, CA 92697, USA.
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MeSH Terms
Antigens, CD34 / metabolism
Antigens, Differentiation / metabolism
Endothelial Cells / cytology*,  metabolism,  ultrastructure
Hepatic Stellate Cells / cytology*,  metabolism,  ultrastructure
Hepatocytes / cytology*,  metabolism,  ultrastructure
Kupffer Cells / cytology*,  metabolism,  ultrastructure
Liver / cytology*,  metabolism,  ultrastructure
Mice, Inbred BALB C
Mice, Inbred ICR
Microscopy, Electron
Nerve Tissue Proteins / metabolism
Grant Support
Reg. No./Substance:
0/Antigens, CD34; 0/Antigens, Differentiation; 0/Nerve Tissue Proteins; 0/glial fibrillary astrocytic protein, mouse; 0/monocyte-macrophage differentiation antigen

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