Document Detail


Cellular observations enabled by microculture: paracrine signaling and population demographics.
MedLine Citation:
PMID:  20011455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cellular microenvironment plays a critical role in shaping and directing the process of communication between the cells. Soluble signals are responsible for many cellular behaviors such as cell survival, proliferation and differentiation. Despite the importance of soluble signals, canonical methods are not well suited to the study of soluble factor interactions between multiple cell types. Macro-scale technology often puts cells into a convective environment that can wash away and dilute soluble signals from their targets, minimizing local concentrations of important factors. In addition, current methods such as transwells, require large numbers of cells and are limited to studying just two cell types. Here, we present data supporting the use of microchannels to study soluble factor signaling providing improved sensitivity as well as the ability to move beyond existing co-culture and conditioned medium paradigms. In addition, we present data suggesting that microculture can be used to unmask effects of population demographics. In this example the data support the hypothesis that a growth promoting subpopulation of cells exists in the mouse mammary gland.
Authors:
Maribella Domenech; Hongmei Yu; Jay Warrick; Nisha M Badders; Ivar Meyvantsson; Caroline M Alexander; David J Beebe
Related Documents :
15239955 - The pdgf/vegf receptor controls blood cell survival in drosophila.
10657695 - Calcium dynamics of neocortical ventricular zone cells.
15719365 - T cell homeostasis requires g protein-coupled receptor-mediated access to trophic signa...
16864795 - Robust, bistable patterning of the dorsal surface of the drosophila embryo.
7531415 - Detection of apoptosis in leukemic and breast cancer cells with monoclonal antibody to ...
3945725 - The in vitro radiobiology of astatine-211 decay.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  1     ISSN:  1757-9708     ISO Abbreviation:  Integr Biol (Camb)     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-12-21     Completed Date:  2010-03-10     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  267-74     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Line
Coculture Techniques / instrumentation*,  methods*
Equipment Design
Equipment Failure Analysis
Humans
Macrophages / cytology,  metabolism*
Male
Microfluidic Analytical Techniques / instrumentation*,  methods*
Paracrine Communication*
Prostatic Neoplasms / metabolism*,  pathology
Grant Support
ID/Acronym/Agency:
5T15LM007359/LM/NLM NIH HHS; K25 CA104162/CA/NCI NIH HHS; K25 CA104162/CA/NCI NIH HHS; K25 CA104162-05/CA/NCI NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  NORMAL FATTY ACID CONCENTRATIONS IN YOUNG CHILDREN WITH PHENYLKETONURIA (PKU).
Next Document:  Comparison of CAT Item Selection Criteria for Polytomous Items.