Document Detail

Cellular observations enabled by microculture: paracrine signaling and population demographics.
MedLine Citation:
PMID:  20011455     Owner:  NLM     Status:  MEDLINE    
The cellular microenvironment plays a critical role in shaping and directing the process of communication between the cells. Soluble signals are responsible for many cellular behaviors such as cell survival, proliferation and differentiation. Despite the importance of soluble signals, canonical methods are not well suited to the study of soluble factor interactions between multiple cell types. Macro-scale technology often puts cells into a convective environment that can wash away and dilute soluble signals from their targets, minimizing local concentrations of important factors. In addition, current methods such as transwells, require large numbers of cells and are limited to studying just two cell types. Here, we present data supporting the use of microchannels to study soluble factor signaling providing improved sensitivity as well as the ability to move beyond existing co-culture and conditioned medium paradigms. In addition, we present data suggesting that microculture can be used to unmask effects of population demographics. In this example the data support the hypothesis that a growth promoting subpopulation of cells exists in the mouse mammary gland.
Maribella Domenech; Hongmei Yu; Jay Warrick; Nisha M Badders; Ivar Meyvantsson; Caroline M Alexander; David J Beebe
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  1     ISSN:  1757-9708     ISO Abbreviation:  Integr Biol (Camb)     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-12-21     Completed Date:  2010-03-10     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  267-74     Citation Subset:  IM    
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MeSH Terms
Cell Line
Coculture Techniques / instrumentation*,  methods*
Equipment Design
Equipment Failure Analysis
Macrophages / cytology,  metabolism*
Microfluidic Analytical Techniques / instrumentation*,  methods*
Paracrine Communication*
Prostatic Neoplasms / metabolism*,  pathology
Grant Support
5T15LM007359/LM/NLM NIH HHS; K25 CA104162/CA/NCI NIH HHS; K25 CA104162/CA/NCI NIH HHS; K25 CA104162-05/CA/NCI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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