Document Detail

Cellular and morphological changes during neointimal hyperplasia development in a porcine arteriovenous graft model.
MedLine Citation:
PMID:  17602194     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Implantation of a haemodialysis arteriovenous graft is often followed by the development of neointimal hyperplasia (NH) at the venous anastomosis. The nature of the proliferating cells in these lesions is not well understood. A better understanding of the cells contributing to NH is important to the development of preventive strategies. METHODS: Carotid-jugular PTFE grafts were placed in 21 pigs and characterized at various time points following implantation. Venous anastomotic tissues were harvested at 1, 7, 14, 21, 28 or 49 post-operative days for histology and immunohistochemistry. RESULTS: Van Gieson staining of the tissues showed that NH was apparent as early as day 7 and progressed over time. Even by day 1, there were cells expressing the proliferation marker Ki-67 in the venous adventitia, but not the media, at the anastomosis. Double immunohistochemical staining showed that these cells were positive for alpha-smooth muscle actin (alpha-SMA), but negative for smooth muscle myosin heavy chain (SM MHC), suggesting that the proliferating cells were myofibroblasts rather than smooth muscle cells. By day 7, proliferating cells were abundant in the adventitia and began to appear in the media, surrounded by extracellular matrix visualized using Trichrome staining. By day 49, alpha-SMA-positive, SM MHC-negative cells were predominant in the NH, and Ki-67 staining had largely vanished. CONCLUSIONS: These results are consistent with the hypothesis that adventitial fibroblasts are transformed into myofibroblasts and begin to proliferate within hours after graft placement. Migration of these cells towards the vessel lumen with subsequent proliferation appears to be a major contributor to NH formation. The pivotal role of the adventitial fibroblasts in the pathogenesis of NH provides a compelling rationale for therapies that target the transformation, proliferation and migration of these cells to prevent arteriovenous graft stenosis.
Li Li; Christi M Terry; Donald K Blumenthal; Tadashi Kuji; Takahisa Masaki; Bonnie C H Kwan; Ilya Zhuplatov; John K Leypoldt; Alfred K Cheung
Related Documents :
16202204 - Interstitial cell of cajal-like cells in the upper urinary tract.
16944774 - Inhibitory effects of saponins from anemarrhena asphodeloides bunge on the growth of va...
7018504 - Testosterone inhibits prostacyclin production by rat aortic smooth muscle cells in cult...
573444 - Action potentials can propagate along small strands of smooth muscle.
16054684 - Porcine-derived xenogeneic bone/poly(glycolide-co-lactide-co-caprolactone) composite an...
11013434 - In vitro development of porcine nuclear transfer embryos constructed using fetal fibrob...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-06-30
Journal Detail:
Title:  Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association     Volume:  22     ISSN:  0931-0509     ISO Abbreviation:  Nephrol. Dial. Transplant.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-30     Completed Date:  2008-03-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8706402     Medline TA:  Nephrol Dial Transplant     Country:  England    
Other Details:
Languages:  eng     Pagination:  3139-46     Citation Subset:  IM    
Department of Medicine, University of Utah, Salt Lake City, UT 84112, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carotid Arteries / pathology*,  surgery*
Cell Division
Jugular Veins / pathology*,  surgery*
Kidney Transplantation / methods*,  pathology
Models, Animal
Tunica Intima / pathology*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Analysis of the factors conditioning the diffusion of peritoneal dialysis in Italy.
Next Document:  Aldosterone induces CTGF in mesangial cells by activation of the glucocorticoid receptor.