Document Detail


Cellular mechanisms of Cl- transport in trout gill mitochondrion-rich cells.
MedLine Citation:
PMID:  19211727     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have studied Cl(-) transport mechanisms in freshwater rainbow trout gill mitochondrion-rich (MR) cells using intracellular pH (pH(i)) imaging. Scanning electron microscopy demonstrated maintenance of cellular polarity in isolated MR cells. MR cell subtypes were identified by Na(+) introduction to the bath, and Cl(-) transport mechanisms were subsequently examined. Cl(-)-free exposure resulted in an alkalinization of pH(i) in both MR cell subtypes, which was dependent on HCO(3)(-) in the bath and inhibited by 1 mM DIDS. Recovery of pH(i) from an acidified state in Na(+)-free conditions was also DIDS sensitive. These results are the first functional evidence for Cl(-)/HCO(3)(-) exchangers in fish gill MR cells. A direct switch from NaCl to Cl(-)-free conditions caused a pH(i) acidification in a subset of MR cells, which was enhanced in the absence of HCO(3)(-). The acidification was replaced by an alkalinization when Cl(-) removal was performed in the presence of NPPB (500 microM) or EIPA (500 microM). Finally, we found that the Na(+)-induced alkalinization of pH(i) found in a previous study is inhibited by EIPA. This inhibitor profile's results suggest the presence of a Cl(-)-dependent Na(+)/H(+) exchange mechanism.
Authors:
Scott K Parks; Martin Tresguerres; Greg G Goss
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-02-11
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  296     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-27     Completed Date:  2009-06-05     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R1161-9     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada. skparks@ualberta.ca
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MeSH Terms
Descriptor/Qualifier:
Animals
Bicarbonates / metabolism
Biological Transport
Cell Polarity
Chloride-Bicarbonate Antiporters / metabolism
Chlorides / metabolism*
Fish Proteins / metabolism
Fresh Water
Gills / cytology,  drug effects,  metabolism*,  ultrastructure
Hydrogen-Ion Concentration
Membrane Transport Modulators / pharmacology
Microscopy, Electron, Scanning
Mitochondria / drug effects,  metabolism*,  ultrastructure
Oncorhynchus mykiss / metabolism*
Sodium Chloride / metabolism
Sodium-Hydrogen Antiporter / metabolism
Time Factors
Grant Support
ID/Acronym/Agency:
GM62328/GM/NIGMS NIH HHS; NS55255/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Bicarbonates; 0/Chloride-Bicarbonate Antiporters; 0/Chlorides; 0/Fish Proteins; 0/Membrane Transport Modulators; 0/Sodium-Hydrogen Antiporter; 7647-14-5/Sodium Chloride
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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