Document Detail

Cellular localization of thrombomodulin in human epithelium and squamous malignancies.
MedLine Citation:
PMID:  7717460     Owner:  NLM     Status:  MEDLINE    
Thrombomodulin is a cell surface glycoprotein that functions as an anticoagulant. Although initially identified on endothelial cells, thrombomodulin is also expressed by other vascular cells, by mesothelial cells, and by epidermal keratinocytes. To determine whether thrombomodulin is expressed by epithelial cells in locations other than skin, we conducted a survey of thrombomodulin protein and mRNA in human epithelium. Thrombomodulin protein was detected by immunohistochemistry in all samples containing stratified squamous epithelium, including oral mucosa, larynx, esophagus, uterine ectocervix, and vagina. In these tissues, thrombomodulin staining localized to the suprabasal layer, with minimal staining observed in the basal or superficial layers of epithelium. Thrombomodulin was not detected in cuboidal, simple columnar, or pseudostratified columnar epithelium and was detected variably in transitional epithelium. Thrombomodulin staining was also observed in 21 of 26 cases of invasive squamous cell carcinoma and in several examples of squamous carcinoma-in-situ and squamous metaplasia. Expression of thrombomodulin mRNA was confirmed by in situ hybridization in both normal and malignant squamous epithelium. Full-length, functionally active thrombomodulin was demonstrated in cultured squamous epithelial cells. These data demonstrate that thrombomodulin expression correlates with the squamous phenotype and suggest that hemostasis is regulated by compartmentalization of procoagulant and anti-coagulant epithelial proteins.
D J Lager; E J Callaghan; S F Worth; T J Raife; S R Lentz
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of pathology     Volume:  146     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-05-12     Completed Date:  1995-05-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  933-43     Citation Subset:  AIM; IM    
Department of Pathology, University of Iowa College of Medicine, Iowa City, USA.
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MeSH Terms
Blotting, Northern
Carcinoma, Squamous Cell / chemistry
Carcinoma, Transitional Cell / chemistry
Cells, Cultured
Epithelium / chemistry*
Immunoenzyme Techniques
In Situ Hybridization
Neoplasms, Squamous Cell / chemistry*
Thrombomodulin / analysis*
Grant Support
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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