| Cellular localization of predicted transmembrane and soluble chemoreceptors in Sinorhizobium meliloti. | |
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MedLine Citation:
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PMID: 19617359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacterial chemoreceptors primarily locate in clusters at the cell pole, where they form large sensory complexes which recruit cytoplasmic components of the signaling pathway. The genome of the soil bacterium Sinorhizobium meliloti encodes seven transmembrane and two soluble chemoreceptors. We have investigated the localization of all nine chemoreceptors in vivo using genome-encoded fusions to a variant of the enhanced green fluorescent protein and to monomeric red fluorescent protein. Six of the transmembrane (McpT to McpX and McpZ) and both soluble (McpY and IcpA) receptors localize to the cell pole. Only McpS, encoded from the symbiotic plasmid pSymA, is evenly distributed in the cell. While the synthesis of all polar localized receptors is confined to exponential growth correlating with the motility phase of cells, McpS is only weakly expressed throughout cell culture growth. Therefore, motile S. meliloti cells form one major chemotaxis cluster that harbors all chemoreceptors except for McpS. Colocalization and deletion analysis demonstrated that formation of polar foci by the majority of receptors is dependent on other chemoreceptors and that receptor clusters are stabilized by the presence of the chemotaxis proteins CheA and CheW. The transmembrane McpV and the soluble IcpA localize to the pole independently of CheA and CheW. However, in mutant strains McpV formed delocalized polar caps that spread throughout the cell membrane while IcpA exhibited increased bipolarity. Immunoblotting of fractionated cells revealed that IcpA, which lacks any hydrophobic domains, nevertheless is associated to the cell membrane. |
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Authors:
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Veronika M Meier; Birgit E Scharf |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-17 |
Journal Detail:
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Title: Journal of bacteriology Volume: 191 ISSN: 1098-5530 ISO Abbreviation: J. Bacteriol. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-28 Completed Date: 2009-09-17 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 5724-33 Citation Subset: IM |
Affiliation:
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Lehrstuhl für Genetik, Universität Regensburg, D-93040 Regensburg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics,
metabolism* Cell Membrane / metabolism Cell Polarity* Chemotaxis* Gene Deletion Gene Expression Regulation, Bacterial Green Fluorescent Proteins / genetics, metabolism Immunoblotting Luminescent Proteins / genetics, metabolism Membrane Proteins / genetics, metabolism* Microscopy, Fluorescence Sinorhizobium meliloti / genetics, growth & development, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Luminescent Proteins; 0/Membrane Proteins; 0/enhanced green fluorescent protein; 0/methyl-accepting chemotaxis proteins; 0/red fluorescent protein; 147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
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