Document Detail


Cellular localization of orexins in human anterior pituitary.
MedLine Citation:
PMID:  14574580     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Orexins A and B are hypothalamic peptides derived from a precursor called prepro-orexin and are associated with the stimulation of food intake and arousal. There is evidence that orexins act on some pituitary functions. Since no studies have been done concerning the presence of orexins in human pituitary, it is unclear whether the local effect of these peptides is due to orexins synthesized in the pituitary or to circulating-derived orexins. To define a possible paracrine regulatory role of orexins on pituitary cell function, we have sought to characterize the expression of orexins in the human adenohypophysis as well as to identify the cell types that express these proteins. In the present study, we used immunohistochemistry and double labeling to detect the presence of orexin A and orexin B in human pituitary. Orexin A was localized in 33% of pituitary cells. With double immunofluorescence techniques we demonstrated that orexin A is present in PRL (82 +/- 5.3%), TSH (18 +/- 2.3%), GH (10 +/- 2.3%), FSH (8 +/- 2.6%), and LH (7 +/- 3.2%) cells, but not in corticotroph cells. Orexin B was found in virtually all corticotrophs cells of the anterior pituitary. These results demonstrate that lactotroph cells are the main source of orexin A and corticotroph cells of orexin B. In summary the present findings provide the first evidence that orexins A and B are expressed in specific human pituitary cell types. Our data provide the cellular basis for a paracrine role of orexins in human pituitary cell function and further our understanding regarding the mechanisms by which orexins influence neuroendocrine function.
Authors:
Montserrat Blanco; Rosalía Gallego; Tomás García-Caballero; Carlos Diéguez; Andrés Beiras
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-08-28
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  120     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-23     Completed Date:  2004-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  259-64     Citation Subset:  IM    
Affiliation:
Department of Morphological Sciences, School of Medicine and University Clinical Hospital, University of Santiago de Compostela, C/ San Francisco, 15782 Santiago de Compostela, Spain.
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MeSH Terms
Descriptor/Qualifier:
Aged
Carrier Proteins / analysis*,  immunology,  metabolism
Female
Humans
Immunohistochemistry
Intracellular Signaling Peptides and Proteins*
Male
Middle Aged
Neuropeptides / analysis*,  immunology,  metabolism
Pituitary Gland, Anterior / chemistry*,  metabolism
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Neuropeptides; 0/orexins

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