Document Detail

Cellular interaction regulates interleukin-8 secretion by granulosa-lutein cells and monocytes/macrophages.
MedLine Citation:
PMID:  19086996     Owner:  NLM     Status:  MEDLINE    
PROBLEM: Peri-ovulatory migration of leukocytes towards the follicle plays an important role during corpus luteum formation. In this study, we examined the secretion of the neutrophil chemoattractant interleukin (IL)-8 by ovarian GL cells and the role of monocytes in IL-8 secretion. METHOD OF STUDY: Granulosa-lutein cells were isolated from the pre-ovulatory follicle. After depletion of contaminating leukocytes, GL cells were co-cultured with the myelo-monocytic cell line THP-1. Intracellular IL-8 accumulation, IL-8 secretion, and chemotactic activity of cell culture media were examined. RESULTS: Intracellular IL-8 was predominantly localized in the endoplasmatic reticulum-Golgi both in GL cells and in THP-1 cells. In co-cultured cells, intracellular IL-8-specific immunofluorescence and IL-8 secretion were increased compared with either GL cells or THP-1 cells that were cultured alone. Conditioned cell culture media from GL cells and THP-1 cells induced directed cell migration by neutrophils. CONCLUSION: Human GL cells constitutively synthesize IL-8. An increased IL-8 secretion by co-cultured GL cells and THP-1 cells suggest that GL cells and monocytes mutually induce chemokine secretion. An initial interaction between GL cells and ovarian leukocytes may therefore contribute to an increased chemokine release and leukocyte recruitment to the forming corpus luteum.
Anna Połeć; Tom Tanbo; Peter Fedorcsák
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of reproductive immunology (New York, N.Y. : 1989)     Volume:  61     ISSN:  1600-0897     ISO Abbreviation:  Am. J. Reprod. Immunol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-17     Completed Date:  2009-02-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8912860     Medline TA:  Am J Reprod Immunol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  85-94     Citation Subset:  IM    
Division of Obstetrics and Gynecology, Rikshospitalet Medical Center, Oslo, Norway.
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MeSH Terms
Cells, Cultured
Coculture Techniques
Interleukin-8 / secretion*
Luteal Cells / secretion*
Macrophages / secretion*
Monocytes / secretion*
Neutrophils / cytology
Reg. No./Substance:

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