| Cellular inhibitor of apoptosis (cIAP1) is down-regulated during retinal ganglion cell (RGC) maturation. | |
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MedLine Citation:
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PMID: 20831867 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptosis, is the main type of cell death that occurs in ageing and neurodegenerative disease, such as glaucoma. This study therefore characterises the expression profile of caspases (pro-apoptosis) and inhibitors of apoptosis (IAPs; anti-apoptosis) during maturation of the Brown Norway rat retina between 6 weeks and >24 weeks and also examines concomitant changes in expression of tumor necrosis factor receptor associated factor 2 (TRAF2). The expression profiles of caspases (initiator caspases 8, 9 and effector caspases 6, 7, 3) and inhibitors of apoptosis (IAPs) (Neuronal IAP), cellular IAP1 and 2 (cIAP1/2), X-chromosome linked IAP (XIAP), Survivin, Bruce and Livin) were examined in retinae from 6 weeks and >24 weeks old BN rats using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR, Western blotting and immunofluoroscence analysis. Caspase expression was not altered significantly during the study interval. IAP expression showed a general reduction during maturation of BN retina, which was statistically significant for cIAP1. cIAP1 reduction was confirmed by Western blotting and immunoflouroscence and was restricted to cells in the retinal ganglion cell layer (RGCL). Accumulation of TRAF2 was observed in the RGCL accompanying the down-regulation of cIAP1 observed. Our results suggest that cells in the mature RGCL may have a greater susceptibility to cell death compared to their younger counterparts and this may be due in part to a reduction in activation of survival pathways involving IAPs and TRAFs. |
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Authors:
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Lilian Kisiswa; Julie Albon; James E Morgan; Michael A Wride |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-08 |
Journal Detail:
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Title: Experimental eye research Volume: 91 ISSN: 1096-0007 ISO Abbreviation: Exp. Eye Res. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-22 Completed Date: 2010-11-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370707 Medline TA: Exp Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 739-47 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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School of Optometry and Vision Sciences, Cardiff University, Maindy Road, Cardiff, CF24 4LU Wales, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Caspases / genetics, metabolism Down-Regulation / physiology* Fluorescent Antibody Technique, Indirect Inhibitor of Apoptosis Proteins / genetics*, metabolism Male Microtubule-Associated Proteins / genetics, metabolism RNA, Messenger / genetics Rats Rats, Inbred BN Retinal Ganglion Cells / metabolism* Reverse Transcriptase Polymerase Chain Reaction TNF Receptor-Associated Factor 2 / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Birc5 protein, rat; 0/Inhibitor of Apoptosis Proteins; 0/Microtubule-Associated Proteins; 0/RNA, Messenger; 0/TNF Receptor-Associated Factor 2; EC 3.4.22.-/Caspases |
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