Document Detail


Cellular immune response in the small intestine of two broiler chicken lines orally inoculated with malabsorption syndrome homogenates.
MedLine Citation:
PMID:  11867167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied the cellular immune response against malabsorption syndrome (MAS) in two broiler chicken lines, A and B. We determined the number of pan T-lymphocytes (CD3), helper T-lymphocytes (CD4), cytotoxic T-lymphocytes (CD8) and macrophages/monocytes in the small intestine in the first 2 weeks after oral inoculation of two MAS homogenates, MAS80 and MAS97-1. The immune cells were detected on cryostat tissue by immunohistochemistry and counted by villus area. In trial 1, we compared the two broiler lines for weight gain depression, intestinal lesion and number of CD3, CD4, CD8 cells and macrophages/monocytes after MAS80 inoculation. Although there was no significant difference in weight gain depression between the two broiler lines, line B had significantly higher numbers of CD8+ T-cells per villus area than had line A. To confirm part of the results of trial 1, trial 2 was done in which we compared different homogenates in broiler line B. Broiler line B was orally inoculated with either MAS97-1, intestinal homogenate obtained from healthy chickens (healthy homogenate), or phosphate buffered saline (PBS). In this trial, the MAS97-1 homogenate also induced weight gain depression and intestinal lesions, whereas the "healthy homogenate" and PBS did not induce weight gain depression or intestinal lesions. The broilers inoculated with MAS97-1 homogenate had significantly more CD8+ T-cells per villus area than had broilers inoculated with "healthy homogenate" or PBS. Increased CD8+ T-cells per villus area in the affected small intestines of broilers suggests an increase of cytotoxic T-cell activity.
Authors:
Th Songserm; B Engel; D J van Roozelaar; G L Kok; A Pijpers; J M A Pol; A A H M ter Huurne
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Veterinary immunology and immunopathology     Volume:  85     ISSN:  0165-2427     ISO Abbreviation:  Vet. Immunol. Immunopathol.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-02-27     Completed Date:  2002-04-04     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8002006     Medline TA:  Vet Immunol Immunopathol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  51-62     Citation Subset:  IM    
Affiliation:
ID-Lelystad BV, Institute for Animal Science and Health, P.O. Box 65, Edelhertweg 15, 8200 AB, Lelystad, The Netherlands. fvettss@ku.ac.th
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD3 / analysis
Body Weight
CD4-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / immunology
Chickens / immunology*
Immunity, Cellular
Intestine, Small / immunology*,  pathology
Killer Cells, Natural / immunology
Macrophages / physiology
Malabsorption Syndromes / immunology,  veterinary*
Monocytes / physiology
Poultry Diseases / immunology*
Chemical
Reg. No./Substance:
0/Antigens, CD3

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