Document Detail


Cellular features of the extra-embryonic endoderm during elongation in the ovine conceptus.
MedLine Citation:
PMID:  17987664     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The extraembryonic endoderm of the elongating ovine conceptus was analyzed by scanning and transmission electron microscopy and by whole mount actin staining and immunofluorescence. Morphological and functional differences between the visceral endoderm (VE), the founding cell layer, and the parietal endoderm (PE) are presented. During the elongation process, the PE differentiated to fusiform multinucleated cells aligned parallel to the elongation axis of the conceptus, whereas the VE cells retained the aspect of typical epithelial cells. The multinucleated PE cells however, expressed cellular and nuclear markers typical of endodermal and polarized epithelial cells. The proteins of the extracellular matrix, laminin, and fibronectin, were specifically expressed in the PE. The presence of pairs of nuclei linked by mid-bodies positively stained with tubulin antibodies, indicated that the syncytial differentiation of the PE was due to karyokinesis which was not followed by cytokinesis rather than by cell fusion.
Authors:
Jacques-E Fléchon; Bernadette Fléchon; Jeril Degrouard; Michel Guillomot
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  45     ISSN:  1526-954X     ISO Abbreviation:  Genesis     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-11-12     Completed Date:  2008-04-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  709-15     Citation Subset:  IM    
Copyright Information:
(c) 2007 Wiley-Liss, Inc.
Affiliation:
Unité Biologie du Développement et Reproduction, Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Embryo, Mammalian / embryology*
Endoderm / embryology*,  metabolism,  ultrastructure
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Sheep / embryology*,  metabolism
Trophoblasts / ultrastructure
Chemical
Reg. No./Substance:
0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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