| Cellular and extracellular matrix changes in anterior cruciate ligaments during human knee aging and osteoarthritis. | |
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MedLine Citation:
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PMID: 23406989 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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ABSTRACT: INTRODUCTION: Anterior cruciate ligament (ACL) degeneration is observed in most osteoarthritis (OA)-affected knee joints. However, the specific spatial and temporal relationship of these changes and their association with extracellular matrix (ECM) degeneration are not well understood. The objective of this study was to characterize the patterns and relationships of aging-related and OA-associated changes in ACL cells and ECM. METHODS: Human knee joints from 80 donors (age 23-94) were obtained at autopsy. ACL degeneration was assessed histologically using a quantitative scoring system. Tissue sections were analyzed for cell density, cell organization, ECM components, ECM-degrading enzymes and markers of differentiation, proliferation, and stem cells. RESULTS: Total cell number in normal ACL decreased with aging but increased in degenerated ACL, due to the formation of perivascular cell aggregates and islands of chondrocyte-like cells. Matrix metalloproteinase (MMP)-1, -3 and MMP-13 expression was reduced in aging ACL but increased in degenerated ACL, mainly in the chondrocyte-like cells. Collagen I was expressed throughout normal and degenerated ACL. Collagen II and X were only detected in the areas with chondroid metaplasia, which also expressed collagen III. Sox9, Runt-related transcription factor 2 (Runx2), and Scleraxis expression was increased in the chondrocyte-like cells in degenerated ACL. Alpha-smooth muscle actin (alpha-SMA), a marker of myofibroblasts and the progenitor cell marker STRO-1 decreased with aging in normal ACL. In degenerated ACL, the new cell aggregates were positive for alpha-SMA and STRO-1. CONCLUSIONS: ACL aging is characterized by reduced cell density and activation. In contrast, ACL degeneration is associated with cell recruitment or proliferation, including progenitor cells or myofibroblasts. Abnormally differentiated chondrocyte-like cell aggregates in degenerated ACL produce abnormal ECM and may predispose to mechanical failure. |
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Authors:
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Akihiko Hasegawa; Hiroyuki Nakahara; Mitsuo Kinoshita; Hiroshi Asahara; James Koziol; Martin K Lotz |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-2-14 |
Journal Detail:
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Title: Arthritis research & therapy Volume: 15 ISSN: 1478-6362 ISO Abbreviation: Arthritis Res. Ther. Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-2-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101154438 Medline TA: Arthritis Res Ther Country: - |
Other Details:
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Languages: ENG Pagination: R29 Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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