| Cellular expression of rheumatoid factor cross-reactive idiotype in patients with seropositive and seronegative rheumatoid arthritis. | |
| | |
MedLine Citation:
|
PMID: 3266364 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A study has been made of the presence of the major rheumatoid Factor cross-reacting idiotype (RCRI) in the serum of patients with seropositive and seronegative rheumatoid arthritis as well as in the cytoplasm of plasmacells induced by cultivation of peripheral blood leucocytes (PBL) from the same RA patients and from normal humans. The PBL were stimulated in vitro with two different B cell mitogens, namely pokeweed mitogen (PWM) or Staph Auerus Cowan strain I (SAC). The RCRI was detected in the serum of seropositive RA patients and in the serum of four out of twenty seronegative RA patients. In these four, however, the seronegative condition was only apparent, since all four had hidden rheumatoid factors that could be shown after dissociation from IgG. The expression of the RCRI in the cytoplasm of PWM-stimulated PBL from seronegative RA patients was concordant with the expression in the serum. Thus the majority of truly seronegative RA patients also appear not to express the RCRI neither in the serum nor in the cytoplasm of plasmacells induced by PWM stimulation of PBL. An unexpected finding was the detection of the RCRI in plasmacells obtained by SAC stimulation of PBL from normal individuals. Up to 40% of RCRI-positive plasmacells were seen in many of these cultures, as opposed to an average of 2% in cultures stimulated with PWM from PBL of the same individuals. High percentages of RCRI-positive plasma-cells have previously been detected by us in PWM cultures only if these were from PBL from RA patients. Our working hypothesis is that PWM is a non-selective stimulator of B cells and that RCRI + precursors are in high frequency in RA patients, whereas SAC selectively stimulates these precursors even in normal PBL populations, where their frequency is not abnormally high. |
| | |
Authors:
|
B Pernis; V R Bonagura |
Related Documents
:
|
16053944 - Induction of profound hypothermia modulates the immune/inflammatory response in a swine... 3872744 - Assessment of circulating immune complexes by a solid-phase c1q-binding assay during th... 22376194 - Intensive aerobic cycling training with lower limb weights in chinese patients with chr... 9000304 - Impact of physical activity and sport on the immune system. 8983924 - The topography of eccrine sweating in humans during exercise. 11283444 - Cardiovascular, metabolic, and hormonal parameters in professional tennis players. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Scandinavian journal of rheumatology. Supplement Volume: 75 ISSN: 0301-3847 ISO Abbreviation: Scand. J. Rheumatol. Suppl. Publication Date: 1988 |
Date Detail:
|
Created Date: 1989-05-11 Completed Date: 1989-05-11 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0400360 Medline TA: Scand J Rheumatol Suppl Country: SWEDEN |
Other Details:
|
Languages: eng Pagination: 66-75 Citation Subset: IM |
Affiliation:
|
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Aged, 80 and over Arthritis, Rheumatoid / blood*, diagnosis, pathology B-Lymphocytes / drug effects, physiology Cross Reactions Female Humans Immunoglobulin Idiotypes / immunology* Lymphocyte Activation Male Middle Aged Pokeweed Mitogens / pharmacology Rheumatoid Factor / analysis, immunology* Serologic Tests Staphylococcus aureus / physiology |
| Grant Support | |
ID/Acronym/Agency:
|
1R01 AM 31936/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Immunoglobulin Idiotypes; 0/Pokeweed Mitogens; 9009-79-4/Rheumatoid Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Recombinant interleukin 1 and tumor necrosis factor acting in synergy to release thromboxane, 6-KETO...
Next Document: Are idiotypic peptides from variable domains critical for T-dependent production of rheumatoid facto...