Document Detail


Cellular expression of gH confers resistance to herpes simplex virus type-1 entry.
MedLine Citation:
PMID:  12890617     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Entry of herpes simplex virus-1 (HSV-1) into cells requires a concerted action of four viral glycoproteins gB, gD, and gH-gL. Previously, cell surface expression of gD had been shown to confer resistance to HSV-1 entry. To investigate any similar effects caused by other entry glycoproteins, gB and gH-gL were coexpressed with Nectin-1 in Chinese hamster ovary (CHO) cells. Interestingly, cellular expression of gB had no effect on HSV-1(KOS) entry. In contrast, entry was significantly reduced in cells expressing gH-gL. This effect was further analyzed by expressing gH and gL separately. Cells expressing gL were normally susceptible, whereas gH-expressing cells were significantly resistant. Further experiments suggested that the gH-mediated interference phenomenon was not specific to any particular gD receptor and was also observed in gH-expressing HeLa cells. Moreover, contrary to a previous report, gL-independent cell surface expression of gH was detected in stably transfected CHO cells, possibly implicating cell surface gH in the interference phenomenon. Thus, taken together these findings indicate that cellular expression of gH interferes with HSV-1 entry.
Authors:
Perry M Scanlan; Vaibhav Tiwari; Susmita Bommireddy; Deepak Shukla
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virology     Volume:  312     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-31     Completed Date:  2003-09-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  14-24     Citation Subset:  IM    
Affiliation:
Department of Microbiology-Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cell Adhesion Molecules / metabolism
Cell Membrane / metabolism
Cricetinae
Gene Expression
Hela Cells
Herpesvirus 1, Human / physiology*
Humans
Viral Envelope Proteins / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
1K22AI053836-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Viral Envelope Proteins; 0/glycoprotein H, herpes simplex virus type 1; 0/glycoprotein L, Human herpesvirus 1; 0/nectins

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