| Cellular envelope phospholipids from Legionella lytica. | |
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MedLine Citation:
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PMID: 18462396 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The composition of phospholipids from the cellular envelope of Legionella lytica grown on artificial medium was determined by two-dimensional thin-layer chromatography. Phosphatidylcholine, phosphatidylethanolamine, and phosphatidyl-N-monomethylethanolamine were the predominant phospholipids, while diphosphatidylglycerol, phosphatidylglycerol, and phosphatidyl-N,N-dimethylethanolamine were present at low concentrations. A trace amount of lipids carrying glycosyl residues was also observed. The fatty acids and their distribution in individual phospholipids were characterized using liquid chromatography/mass spectrometry (LC/MS), matrix-assisted laser desorption ionization-time of flight, and gas chromatography/MS methods. The characteristic feature of L. lytica phospholipids was the presence of an unbranched chain (which differentiates this bacterium from Legionella pneumophila) and branched iso and anteiso fatty acids as well as cis-9,10-methylenehexadecanoic acid. According to spectroscopic LC/MS data, the localization of saturated and unsaturated fatty acid residues on phosphorylglycerol was determined. Some aspects of the significance of phosphatidylcholine, one of the main phospholipids in L. lytica, are addressed and taxonomic implications of the data are discussed. |
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Authors:
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Marta Palusinska-Szysz; Rafal Kalitynski; Ryszard Russa; Andrzej L Dawidowicz; Wincenty J Drozanski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-06 |
Journal Detail:
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Title: FEMS microbiology letters Volume: 283 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-15 Completed Date: 2008-07-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: England |
Other Details:
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Languages: eng Pagination: 239-46 Citation Subset: IM |
Affiliation:
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Department of General Microbiology, Maria Curie-Sklodowska University, Akademicka St. 19, Lublin, Poland. martasz@biotop.umcs.lublin.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Membrane
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chemistry* Chromatography, Liquid Chromatography, Thin Layer / methods Fatty Acids / analysis, isolation & purification Fatty Acids, Unsaturated / analysis, isolation & purification Gas Chromatography-Mass Spectrometry Legionella / chemistry* Mass Spectrometry Phospholipids / analysis*, isolation & purification Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Phospholipids |
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