Document Detail

Cellular efflux of auxin catalyzed by the Arabidopsis MDR/PGP transporter AtPGP1.
MedLine Citation:
PMID:  16212599     Owner:  NLM     Status:  MEDLINE    
Directional transport of the phytohormone auxin is required for the establishment and maintenance of plant polarity, but the underlying molecular mechanisms have not been fully elucidated. Plant homologs of human multiple drug resistance/P-glycoproteins (MDR/PGPs) have been implicated in auxin transport, as defects in MDR1 (AtPGP19) and AtPGP1 result in reductions of growth and auxin transport in Arabidopsis (atpgp1, atpgp19), maize (brachytic2) and sorghum (dwarf3). Here we examine the localization, activity, substrate specificity and inhibitor sensitivity of AtPGP1. AtPGP1 exhibits non-polar plasma membrane localization at the shoot and root apices, as well as polar localization above the root apex. Protoplasts from Arabidopsis pgp1 leaf mesophyll cells exhibit reduced efflux of natural and synthetic auxins with reduced sensitivity to auxin efflux inhibitors. Expression of AtPGP1 in yeast and in the standard mammalian expression system used to analyze human MDR-type proteins results in enhanced efflux of indole-3-acetic acid (IAA) and the synthetic auxin 1-naphthalene acetic acid (1-NAA), but not the inactive auxin 2-NAA. AtPGP1-mediated efflux is sensitive to auxin efflux and ABC transporter inhibitors. As is seen in planta, AtPGP1 also appears to mediate some efflux of IAA oxidative breakdown products associated with apical sites of high auxin accumulation. However, unlike what is seen in planta, some additional transport of the benzoic acid is observed in yeast and mammalian cells expressing AtPGP1, suggesting that other factors present in plant tissues confer enhanced auxin specificity to PGP-mediated transport.
Markus Geisler; Joshua J Blakeslee; Rodolphe Bouchard; Ok Ran Lee; Vincent Vincenzetti; Anindita Bandyopadhyay; Boosaree Titapiwatanakun; Wendy Ann Peer; Aurèlien Bailly; Elizabeth L Richards; Karin F K Ejendal; Aaron P Smith; Célia Baroux; Ueli Grossniklaus; Axel Müller; Christine A Hrycyna; Robert Dudler; Angus S Murphy; Enrico Martinoia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  44     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-10     Completed Date:  2006-01-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  179-94     Citation Subset:  IM    
Basel-Zurich Plant Science Center, University of Zurich, Institute of Plant Biology, CH-8007 Zurich, Switzerland.
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MeSH Terms
ATP-Binding Cassette Transporters / metabolism*
Arabidopsis / metabolism*
Arabidopsis Proteins / metabolism*
Biological Transport, Active
Gene Expression Regulation, Plant / physiology
Hela Cells
Indoleacetic Acids / metabolism*
Naphthaleneacetic Acids / metabolism
Plant Growth Regulators / metabolism*
Plant Roots / metabolism
Plant Shoots / metabolism
Saccharomyces cerevisiae
Substrate Specificity
Reg. No./Substance:
0/ATP-Binding Cassette Transporters; 0/ATPGP1 protein, Arabidopsis; 0/Arabidopsis Proteins; 0/Indoleacetic Acids; 0/Naphthaleneacetic Acids; 0/Plant Growth Regulators; 86-87-3/1-naphthaleneacetic acid; 87-51-4/indoleacetic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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