Document Detail

Cellular effects of platelet rich plasma: a study on HL-60 macrophage-like cells.
MedLine Citation:
PMID:  17487092     Owner:  NLM     Status:  MEDLINE    
Healing injured tissue in the body is a complex process which consists of four distinct phases: hemostasis, inflammation, proliferation, and remodeling. Each of these phases is coordinated by growth factor release and cell to cell interactions. Platelet rich plasma (PRP) is a fraction of plasma that has been isolated and used to enhance regeneration in bone and soft tissues. The healing potential of PRP has been attributed to the release of multiple growth factors from the highly concentrated platelets. While there is strong evidence of the pro-stimulatory effect on the cellular proliferation phase of healing, there is little evidence of the effects of PRP on the inflammatory phase of healing. In this study we investigate the effect that PRP has on macrophage cells in culture and the implications this has on the healing process. We investigate specifically the effects of the separate cellular components of PRP, as a whole and individually, on cell proliferation in human macrophage cells in culture. In contrast to the pro-stimulatory effect that PRP has on cells such as osteoblasts, fibroblasts, and tendon cells; our results show a suppression ofmacrophages by PRP as early as 24 hours after treatment. This suppression was statistically significant (p=0.002) and continued to be significant for the duration of the study. The cell viability results of PRP compared to platelet poor plasma (PPP) and individual components of PRP showed that PRP resulted in a steady increase in viability following the initial insult to the macrophage cells, while the viability of other treatment groups seemed to plateau.
James Woodall; Michelle Tucci; Allan Mishra; Hamed Benghuzzi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical sciences instrumentation     Volume:  43     ISSN:  0067-8856     ISO Abbreviation:  Biomed Sci Instrum     Publication Date:  2007  
Date Detail:
Created Date:  2007-05-09     Completed Date:  2007-06-13     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0140524     Medline TA:  Biomed Sci Instrum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  266-71     Citation Subset:  IM    
Department of Orthopedic Surgery and Rehabilitation, University of Mississippi Medical Center, 2500 N. State St. Jackson, MS 39216, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Communication / immunology*
Cell Proliferation
Cell Size
Cell Survival
Cells, Cultured
Cytokines / immunology*
Macrophages / cytology*,  immunology*
Platelet-Rich Plasma / cytology*,  immunology*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The effects of sustained delivery of cortisol on kidney tubule epithelial cells in vitro.
Next Document:  A comparison of 5-fluorouracil and natural chemotherapeutic agents, EGCG and thymoquinone, delivered...