Document Detail


Cellular differentiation of basal cell carcinoma studied with fluorescent lectins and cytokeratin antibodies.
MedLine Citation:
PMID:  6084019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The expression of cellular glycoconjugates and cytokeratin polypeptides in 8 basal cell carcinomas (BCC) was studied using fluorochrome-coupled lectins and different keratin-antibodies. Peanut agglutinin and Wistaria floribunda agglutinin, binding to all layers of normal human epidermis, also stained all cells in the basal cell carcinomas. Dolichos biflorus agglutinin, which in normal epidermis binds only to the basal cells, gave a mottled staining pattern in most of the tumors. Instead, Ulex europaeus I agglutinin and soybean agglutinin, which in normal epidermis only bind to the spinous and granular cell layers, did not stain tumor cells in basal cell carcinomas. Rabbit antibodies to human 43-50kD epidermal keratin polypeptides and 2 monoclonal cytokeratin antibodies, PKK1 reacting only with follicular epithelium, and PKK2 reacting also with the basal epidermal cells, brightly stained all cells of the basal cell carcinomas studied, whereas antibodies to human 60-67kD epidermal keratin polypeptides did not bind to the carcinoma cells. The results suggest that the cells in basal cell carcinomas resemble epidermal basal cells both by their glycoconjugate pattern and keratin expression. However, the tumor cells also express cytokeratins, which can be found only in the follicular epithelium, but not in normal interfollicular epidermis.
Authors:
A L Kariniemi; H Holthöfer; T Vartio; I Virtanen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cutaneous pathology     Volume:  11     ISSN:  0303-6987     ISO Abbreviation:  J. Cutan. Pathol.     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-03-13     Completed Date:  1985-03-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0425124     Medline TA:  J Cutan Pathol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  541-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Antibodies / immunology
Carcinoma, Basal Cell / pathology*
Cell Differentiation
Fluorescent Dyes / diagnostic use
Humans
Keratins / immunology
Lectins / diagnostic use
Middle Aged
Skin Neoplasms / pathology*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Fluorescent Dyes; 0/Lectins; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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