| Cellular determinants of low-shear blood viscosity. | |
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MedLine Citation:
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PMID: 9474265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Low-shear viscometry is one of the methods commonly used to estimate the degree of red blood cell (RBC) aggregation in various bloods and RBC suspensions. However, it has been previously shown that alterations in RBC morphology and mechanical behavior can affect the low-shear apparent viscosity of RBC suspensions; RBC aggregation is also sensitive to these cellular factors. This study used heat treatment (48 degrees C, 5 min), glutaraldehyde (0.005-0.02%) and hydrogen peroxide (1 mM) to modify cell geometry and deformability. Red blood cell aggregation was assessed via a Myrenne Aggregometer ("M" and "MI" indexes), RBC suspension viscosity was measured using a Contraves LS-30 viscometer, and RBC shape response to fluid shear stresses (i.e., deformability) was determined by ektacytometry (LORCA system). Our results indicate that low-shear apparent viscosity and related indexes may not always reflect changes of RBC aggregation if cellular properties are altered: for situations where RBC aggregation has been only moderately affected, cellular mechanical factors may be the major determinant of low-shear viscosity. These findings thus imply that in situations which may be associated alterations of RBC geometry and/or deformability, low-shear viscometry should not be the sole measurement technique used to assess RBC aggregation. |
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Authors:
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O K Baskurt; H J Meiselman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biorheology Volume: 34 ISSN: 0006-355X ISO Abbreviation: Biorheology Publication Date: 1997 May-Jun |
Date Detail:
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Created Date: 1998-03-17 Completed Date: 1998-03-17 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0372526 Medline TA: Biorheology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 235-47 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, USC School of Medicine, Los Angeles, California 90033, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blood Viscosity* Erythrocyte Aggregation / drug effects Erythrocyte Deformability / drug effects Female Glutaral / pharmacology Hemorheology* Hot Temperature Humans Hydrogen Peroxide / pharmacology Male Middle Aged |
| Grant Support | |
ID/Acronym/Agency:
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HL15722/HL/NHLBI NIH HHS; HL48484/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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111-30-8/Glutaral; 7722-84-1/Hydrogen Peroxide |
| Comments/Corrections | |
Comment In:
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Biorheology. 1997 Jul-Oct;34(4-5):375-6
[PMID:
9578810
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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