Document Detail


Cellular defense against singlet oxygen-induced oxidative damage by cytosolic NADP+-dependent isocitrate dehydrogenase.
MedLine Citation:
PMID:  12688426     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Singlet oxygen (1O2) is a highly reactive form of molecular oxygen that may harm living systems by oxidizing critical cellular macromolecules. Recently, we have shown that NADP+-dependent isocitrate dehydrogenase is involved in the supply of NADPH needed for GSH production against cellular oxidative damage. In this study, we investigated the role of cytosolic form of NADP+-dependent isocitrate dehydrogenase (IDPc) against singlet oxygen-induced cytotoxicity by comparing the relative degree of cellular responses in three different NIH3T3 cells with stable transfection with the cDNA for mouse IDPc in sense and antisense orientations, where IDPc activities were 2.3-fold higher and 39% lower, respectively, than that in the parental cells carrying the vector alone. Upon exposure to singlet oxygen generated from photoactivated dye, the cells with low levels of IDPc became more sensitive to cell killing. Lipid peroxidation, protein oxidation, oxidative DNA damage and intracellular peroxide generation were higher in the cell-line expressing the lower level of IDPc. However, the cells with the highly over-expressed IDPc exhibited enhanced resistance against singlet oxygen, compared to the control cells. The data indicate that IDPc plays an important role in cellular defense against singlet oxygen-induced oxidative injury.
Authors:
Sun Yee Kim; Jeen-Woo Park
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical research     Volume:  37     ISSN:  1071-5762     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-04-11     Completed Date:  2003-09-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  309-16     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu, South Korea.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Carbon / chemistry
Cell Line
Cell Line, Transformed
Cell-Free System
Cytosol / metabolism*
DNA Damage
DNA, Complementary / metabolism
Deoxyguanosine / analogs & derivatives*,  metabolism
Dose-Response Relationship, Drug
Fluorescent Dyes / pharmacology
Genetic Vectors
Glutathione / metabolism
Humans
Immunoblotting
Isocitrate Dehydrogenase / chemistry*,  metabolism
Lipid Peroxidation
Methylene Blue / pharmacology
Mice
Microscopy, Fluorescence
NADP / metabolism*
Oxidative Stress*
Oxygen / chemistry*,  metabolism*
Proline / analogs & derivatives*,  pharmacology
Proteins / chemistry
Pyridines / pharmacology
Reactive Oxygen Species / metabolism
Rose Bengal / pharmacology
Singlet Oxygen*
Transfection
U937 Cells
Chemical
Reg. No./Substance:
0/8-hydroxy-2'-deoxyguanosine; 0/DNA, Complementary; 0/Fluorescent Dyes; 0/Proteins; 0/Pyridines; 0/Reactive Oxygen Species; 11121-48-5/Rose Bengal; 126706-34-1/4,4-dicarboxy-5-(pyridoxyl-5'-phosphate)proline; 147-85-3/Proline; 17778-80-2/Singlet Oxygen; 53-59-8/NADP; 61-73-4/Methylene Blue; 70-18-8/Glutathione; 7440-44-0/Carbon; 7782-44-7/Oxygen; 961-07-9/Deoxyguanosine; EC 1.1.1.41/Isocitrate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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