| Cellular content of ribonucleic acid and protein in Saccharomyces cerevisiae as a function of exponential growth rate: calculation of the apparent peptide chain elongation rate. | |
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MedLine Citation:
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PMID: 1089627 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The average cellular content of ribonucleic acid and protein was determined in cultures of Saccharomyces cerevisiae growing exponentially at different rates in a variety of media. Estimations of the proportion of total cellular ribonucleic acid that is made up of ribosomal ribonucleic acid were used to calculate the average number of ribosomes per cell at the different growth rates. The fraction of ribosomes actively engaged in translation was estimated by sucrose gradient centrifugation of ribosomes and polysomes. These data were used in a calculation of the apparent time taken for the addition of an amino acid to the growing polypeptide chain; this value was found to vary linearly with growth rate over a fivefold range of doubling times. |
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Authors:
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K W Boehlke; J D Friesen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 121 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1975 Feb |
Date Detail:
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Created Date: 1975-05-10 Completed Date: 1975-05-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 429-33 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine
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metabolism Carbon Radioisotopes Centrifugation, Density Gradient Fungal Proteins / analysis, biosynthesis* Lysine / metabolism Peptide Chain Elongation, Translational* Protein Biosynthesis RNA / analysis, biosynthesis* RNA, Ribosomal / analysis Ribosomes / metabolism Saccharomyces cerevisiae / growth & development*, metabolism Spectrophotometry Tritium Uracil / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Fungal Proteins; 0/RNA, Ribosomal; 10028-17-8/Tritium; 56-87-1/Lysine; 63231-63-0/RNA; 66-22-8/Uracil; 73-24-5/Adenine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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