Document Detail


Cellular content of ribonucleic acid and protein in Saccharomyces cerevisiae as a function of exponential growth rate: calculation of the apparent peptide chain elongation rate.
MedLine Citation:
PMID:  1089627     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The average cellular content of ribonucleic acid and protein was determined in cultures of Saccharomyces cerevisiae growing exponentially at different rates in a variety of media. Estimations of the proportion of total cellular ribonucleic acid that is made up of ribosomal ribonucleic acid were used to calculate the average number of ribosomes per cell at the different growth rates. The fraction of ribosomes actively engaged in translation was estimated by sucrose gradient centrifugation of ribosomes and polysomes. These data were used in a calculation of the apparent time taken for the addition of an amino acid to the growing polypeptide chain; this value was found to vary linearly with growth rate over a fivefold range of doubling times.
Authors:
K W Boehlke; J D Friesen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  121     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1975 Feb 
Date Detail:
Created Date:  1975-05-10     Completed Date:  1975-05-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  429-33     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenine / metabolism
Carbon Radioisotopes
Centrifugation, Density Gradient
Fungal Proteins / analysis,  biosynthesis*
Lysine / metabolism
Peptide Chain Elongation, Translational*
Protein Biosynthesis
RNA / analysis,  biosynthesis*
RNA, Ribosomal / analysis
Ribosomes / metabolism
Saccharomyces cerevisiae / growth & development*,  metabolism
Spectrophotometry
Tritium
Uracil / metabolism
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Fungal Proteins; 0/RNA, Ribosomal; 10028-17-8/Tritium; 56-87-1/Lysine; 63231-63-0/RNA; 66-22-8/Uracil; 73-24-5/Adenine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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