Document Detail


Cellular cohesiveness in benign and malignant thyroid follicular tumours varies significantly, but the difference is not useful in diagnosis of individual cases.
MedLine Citation:
PMID:  21198996     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
T. Rozkos, A. Ryska, J. Cap, F. Sobande and J. Laco Cellular cohesiveness in benign and malignant thyroid follicular tumours varies significantly, but the difference is not useful in diagnosis of individual cases Introduction:  The aim of our study was to search for new, readily available and statistically reliable cytological markers for differentiating benign and malignant follicular thyroid neoplasms pre-operatively. Methods:  Cohesiveness of tumour cells in cytology slides from a series of 58 follicular tumours diagnosed between 1998 and 2004 inclusive was studied, including 48 follicular adenomas, and eight minimally invasive and two widely invasive follicular carcinomas. Photomicrographs of the cytology slides were taken and the digital images were analysed using computer image analysis software. We evaluated the relative proportions of cells arranged in groups of various sizes. The cohesiveness of the cells in cytological smears was then correlated with the immunohistochemical expression of E-cadherin in corresponding histological slides. Results:  Cases from 15 men (26%) and 43 women (74%) with a mean age of 50 years (range, 19-79) were analysed. In follicular adenomas and carcinomas, respectively, isolated cells were seen in 16.8% and 24.7% (P = 0.028), groups of two to five cells in 9.7% and 11.5% (P = 0.145) and groups of more than five cells in 73.5% and 63.8% (P = 0.041). The mean cell count in groups with more than five cells was 46.5 and 27.0 in adenomas and carcinomas, respectively (P < 0.001). Cell cohesiveness, either as percentage of cells in groups of more than five (R(2)  = 0.026) or as mean cell count per group of more than five (R(2)  = 0.005), was not found to be dependent on the expression of E-cadherin. Using a threshold of 13% isolated tumour cells in cytological smears, follicular adenomas and carcinomas could be distinguished with 90% sensitivity and 41% specificity. Conclusions:  Although we demonstrated a statistically significant difference in cell cohesion between follicular adenomas and carcinomas, these could not be distinguished in the clinical setting by evaluation of the percentage of isolated cells in cytological smears because the specificity was too low. The absence of correlation of cellular cohesiveness with E-cadherin expression indicates that other factors are probably responsible for the loss of cohesiveness observed in follicular thyroid malignancy.
Authors:
T Rozkos; A Ryska; J Cap; F Sobande; J Laco
Related Documents :
21323946 - Cd151 expression can predict cancer progression in clear cell renal cell carcinoma.
5335906 - Methionine and the regulation of ribonucleic acid synthesis in escherichia coli.
7439326 - Effect of cell synchronization techniques on polyamine content of hela cells.
130626 - Functional and ultrastructural studies of sezary cells.
11429776 - Growth kinetics of algal populations exsymbiotic from paramecium bursaria by flow cytom...
9344606 - Monitoring uptake of ellipticine and its fluorescence lifetime in relation to the cell ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-28
Journal Detail:
Title:  Cytopathology : official journal of the British Society for Clinical Cytology     Volume:  -     ISSN:  1365-2303     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-1-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010345     Medline TA:  Cytopathology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2010 Blackwell Publishing Ltd.
Affiliation:
The Fingerland Department of Pathology, Charles University, Faculty of Medicine and University Hospital in Hradec Kralove, Czech Republic Department of Internal Medicine II, Charles University, Faculty of Medicine and University Hospital in Hradec Kralove, Czech Republic.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Adiponectin in relation to insulin sensitivity and insulin secretion in the development of type 2 di...
Next Document:  Establishing RHD zygosity in India: a step into the future of foetal and neonatal haemolytic disease...